Inhibition of MicroRNA-29 Enhances Elastin Levels in Cells Haploinsufficient for Elastin and in Bioengineered Vessels
Objective—The goal of this study was to determine whether antagonizing microRNA (miR)-29 enhances elastin (ELN) levels in cells and tissues lacking ELN.
Methods and Results—miR-29 mimics reduced ELN levels in fibroblasts and smooth muscle cells, whereas miR-29 inhibition increased ELN levels. Antagonism of miR-29 also increased ELN levels in cells from patients haploinsufficient for ELN and in bioengineered human vessels.
Conclusion—miR-29 antagonism may promote increased ELN levels during conditions of ELN deficiencies.
- Received September 2, 2011.
- Accepted November 2, 2011.
- © 2011 American Heart Association, Inc.