Abstract 720: Edaravone Reduces Kidney Damage Induced by Myonephropathic Metabolic Syndrome in Rats
Background: Free radicals cause myonephropathic metabolic syndrome (MNMS), which damages not only muscles but also kidneys. At ATVB 2014, we previously reported edaravone (Radicut®, Mitsubishi Tanabe Pharma Co., Japan), the free radical scavenger may suppress kidney damage induced by MNMS.
Objective: In this study, we evaluated whether edaravone suppresses MNMS-induced kidney damage, focusing on active neutrophils and tubular function.
Methods: Either 3.0 mg/kg of edaravone (edaravone group; n = 4), or saline (MNMS group; n = 6) was intraperitoneally injected into male Lewis male rats (508 ± 31 g). MNMS models were induced, by clamping the bilateral common femoral arteries for 5 hours, followed by de-clamping after more 5 hours. The both kidneys were stained with naphthol AS-D chloroacetate esterase to detect active neutrophils and with Periodic Acid Schiff (PAS) to assess glycogen storage. Normal kidneys were also stained as control (n = 3). Kidney damage was evaluated by counting the number of chloroacetate esterase-positive cells (neutrophil infiltration) and the percentage of PAS-positive area (tubular function).
Results: The number of chloroacetate esterase-positive cells was significantly increased in the MNMS group than that in normal kidneys (62.4 ± 5.6 vs. 17.5 ± 2.4 cells/mm2, p<0.01). However, the number of chloroacetate esterase-positive cells was significantly reduced in the edaravone group (32.8 ± 5.7 cells/mm2, p<0.01, Figure attached). The percentage of PAS-positive area in the MNMS group was significantly decreased than that in normal kidneys (36.9 ± 2.6 vs. 66.9 ± 1.2%, p<0.01). The percentage of PAS-positive area was maintained in the edaravone group (52.9 ± 0.9 %, p<0.01, Figure attached).
Conclusions: Edaravone may reduce neutrophil infiltration around the glomerulus and tubular dysfunction induced by MNMS.
Author Disclosures: M. Yamamura: Research Grant; Modest; Grant-in-Aid for Researchers, Hyogo College of Medicine 2013. M. Mitsuno: None. H. Tanaka: None. M. Ryomoto: None. S. Fukui: None. T. Kajiyama: None. Y. Miyamoto: None.
- © 2015 by American Heart Association, Inc.