Abstract 701: Differential Expression of Epicardial Fat MiRNA Targets in Patients with Coronary Artery Disease
Background and Objectives: Adipose dysfunction is a major player in cardiometabolic diseases. Epicardial fat (EF) plays an important role in the pathophysiology of coronary-artery disease (CAD). EF factors have a paracrine and endocrine effect on heart and vasculature. We earlier showed differences in EF-derived biomarkers in patients with and without CAD. The objective of this study was to assess EF miRNA and its target mRNA expression profiles in patients with and without CAD.
Methods: EF was obtained from men and women undergoing coronary-artery-bypass-graft or valve-type surgery (IRB approved study). Total RNA and miRNA were isolated from EF. Whole RNA sequencing (Next-generation sequencing) and whole-genome miRNA array of the EF from all patients was performed.
Results: 31 miRNAs were differentially expressed (24 up and 7 down-regulated) in EF from CAD compared to non-CAD patients. Cross-referencing the target mRNAs of these miRNAs using Ingenuity Pathway Analysis and RNA-seq data, identified genes that played a role in CAD: GRIN1, DUSP4 (ion transport); APOB, LDLR (lipoprotein homeostasis); TUBB1 (platelet function); and FOXO3 (vascular homeostasis).
Conclusion: Our results identified miRNA-mRNA pairs differentially expressed in EF that play a role in CAD. Our future research will focus on validating the functional importance of these miRNA-mRNA pairs in relevance to epicardial fat-vascular cross talk
Author Disclosures: N. Santanam: None. M. Davis: None. D. Primerano: None. T. Gress: None. P. Wehner: None. N. Chowdhury: None.
- © 2015 by American Heart Association, Inc.