Abstract 487: Saturated FFA Induced NF-κB Activation Is Independent of IKK/IκBα in Aortic Endothelial Cells
Accumulating evidence has suggested a linkage between free fatty acids (FFAs) and chronic inflammation. It has been shown that saturated FFAs inhibits insulin signal in adipocytes though the activation of IKK. In this study, we examined the effect of FFAs on NF-κB activation in bovine aortic endothelial cells. We found that the saturated FFAs, but not the unsaturated FFAs, caused the activation of NF-κB at a low but sustained level. As a result, ICAM-1 expression was also increased. Interestingly, inconsistent with the previous reports on insulin signal, no changes in IKK phosphorylation and IκBα protein level were observed. IKK16, an IKK inhibitor, failed to block saturated FFA induced NF-κB nuclear translocation. These results imply that saturated FFA induced NF-κB activation is independent of IKK activation and IκBα degradation in aortic endothelial cells, suggesting a novel mechanism for NF-κB activation.
Author Disclosures: F. Zhang: None. X. Luo: None. L. Wei: None.
- © 2015 by American Heart Association, Inc.