Abstract 457: Mirnas 362-3p, 194 and 9b-1 In Aaa and Popliteal Aneurysm Pathogenesis
Introduction: Abdominal aortic aneurysm (AAA) remains a major challenge in treatment and cause of death in cardiovascular patients. Despite open surgery or endovascular stent exclusion no therapeutic option remains to clinicians. Similar in severity but much less known are popliteal aneurysms (PA) arising from muscular type arteries often resulting in leg ischemia. So far focus has been on atherosclerosis and inflammation involved in this largely unclear pathogenesis as relevant pathways that could be influenced by i.e. medication in order to prevent growth and possible rupture.
Material and methods: miRNA global expression profiles and RT-PCR for confirmation experiments have been used on human tissue samples. DIANA based pathway prediction and immunhistochemical examinations were applied.
Results: miRNAs 362-3p, 194 and 9b-1 revealed by a global expression assays from AAA specimens were found to be differentially regulated between the two aneurysm entities. Computational pathway analysis predicted enrichment in metabolic, ECM and cell-cell-interatcion pathways. Here distinct features were found in immunhistochemical analysis and apoptosis assays.
Conclusion: Further experiments are needed to confirm the involvement of alternative pathway in AAA and PA pathogenesis. The comparison between elastic and muscular type arteries however may result in exclusive aneurysm mechanisms.
Author Disclosures: A. Busch: None. A. Zernecke: None. M. Busch: None. L. Maegdefessel: None. U. Lorenz: None.
- © 2015 by American Heart Association, Inc.