Abstract 244: Arterial Calcification Decreases Functional Recovery in a Murine Model of Hindlimb Ischemia.
Objectives: Clinical studies have demonstrated associations between arterial calcification and adverse outcomes among patients with peripheral arterial disease (PAD). The causal relationship between calcification and the pathophysiology of ischemia, however, remains unclear. This study aims to evaluate the effects of vitamin D3-induced arterial calcification on functional recovery and resolution of ischemia in the rat hindlimb model.
Methods: Arterial calcification was induced in 14 Sprague-Dawley rats using Vitamin D3. A control group of 14 rats received vehicle only. After 2 weeks to allow for calcification, unilateral hindlimb ischemia was induced by ligation and excision of the superficial femoral artery. Functional status and ischemia status were evaluated weekly for 1 month in a blinded manner.
Results: 28 rats were assessed at all designated time periods. Arterial calcification was significantly greater in Vitamin D3-treated animals than in controls. Vitamin D3-treated rats had significantly worse total ischemia scores at each time point compared with controls. (Figure 1). All rats in the control group regained normal functional status and ischemia scores by week 4. Functional and ischemia scores in vitamin D3-treated animals did not return to baseline by the conclusion of the study.
Conclusions: Vitamin D3 treatment significantly reduces functional recovery in a rat model of hindlimb ischemia. Future research aimed at understanding the relationship between vitamin D3 treatment, arterial calcification, and lower extremity ischemia may help to improve outcomes in patients with PAD.
Author Disclosures: S.L. Zettervall: None. S. Monk: None. X. Wang: None. T. Lin: None. R.J. Guzman: None.
- © 2015 by American Heart Association, Inc.