Abstract 167: High Dietary Fructose Does not Exacerbate the Detrimental Consequences of a HF Diet on Basilar Artery Function
There is a strong correlation between the consumption of sugar- or fructose-enriched products and obesity. Moreover, high dietary fructose without added fat and in the absence of obesity induces leptin resistance and exacerbates subsequent HF-induced obesity. Elevated leptin is associated with arterial stiffness. Thus, we hypothesized that the consequences of a high-fat/high-fructose (HF/F) diet would be more detrimental than a high-fat (HF) or chow diet on functional and structural changes in the basilar arteries. Male Sprague Dawley rats were fed either with chow, HF (30%) or HF/F (30%/44%) diets for 12 weeks (N=8-10/group). The basilar artery was cannulated in a pressurized system (90cm H2O) and vascular contractile responses to KCl (30-120 mM) and endothelin (10-11-10-7 M), and relaxation to acetylcholine (ACh) (10-10-10-4 M), and diethylamine (DEA)-NONO-ate (10-10-10-4 M) evaluated and artery wall thickness and eNOS immunofluroescence determined. At death, leptin, blood lipids, and asymetricaldiamethyl arginine level (ADMA) were assayed in serum. Basilar artery contractility was unchanged, but both HF and HF/F diets resulted in decreased relaxation responses compared with chow to endothelium dependent (Ach logEC50: -6.48, chow; -5.65, HF; 5.79HF/F, p<0.05) and endothelium independent relaxation (DEA: -5.94;-4.45;-4.49, p<.05). Increased basilar artery wall thickness was observed only in HF group (22±1.2 vs 27±1.6μm, p<.05). Both diets increased serum ADMA levels (.36±.03 HF; .42±.05 HF/F vs .26±.02 chow, p<.05), 50% decreased eNOS immunofluorescence (arbitrary units, p<.05), and increased adipose tissue (10.7±.5 HF; 12.7±.8 HF/F vs 7.8±.3 chow, p<.05). HF/F increased triglycerides (157±32 mg/dl) and leptin (10.4±1.8ng/ml) greater than with HF (65±15; 6.6±.6) or chow diet (63±14; 4.1±.3). In conclusion, both HF diets impaired basilar artery relaxation, potentially due to decreased eNOS synthesis and increased ADMA. Although, leptin and triglycerides were greater with HF/F diet only the HF increased wall thickness. These data suggest that the presence of high fructose in a HF diet does not exacerbate the detrimental consequences of a HF diet on basilar artery function.
Author Disclosures: N. Tumer: None. H.Z. Toklu: None. J. Muller-Delp: None. Y. Sakarya: None. S. Oktay: None. N. Kirichenko: None. M. Matheny: None. C. Carter: None. D. Morgan: None. K. Strehler: None. P.J. Scarpace: None.
- © 2015 by American Heart Association, Inc.