Abstract 111: HDL-apolipoprotein A-I Exchange Is Impaired in the Metabolic Syndrome
Objective: Metabolic syndrome (MetSyn) is associated with a host of cardiovascular risk factors including increased waist circumference, triglycerides, blood pressure, fasting glucose, and reduced high density lipoprotein cholesterol (HDL-C). Changes in HDL function, especially the ability to participate in reverse cholesterol transport, are more indicative of the atheroprotective properties of HDL compared to HDL-C levels alone. In this study, we determine the association of HDL-apolipoproteinA-I (apoA-I) exchange, a measure of HDL remodeling/apoA-I exchange, with MetSyn and its individual components.
Methods and Results: We analyzed HDL-apoA-I exchange (HAE) in the plasma of 60 well-characterized subjects with MetSyn and 14 healthy control subjects. HAE measurements were obtained using electron paramagnetic resonance (EPR) following incubation of plasma with lipid-freeapoA-I containing a methanethiosulfonate spin label. HAE positively correlated with plasma HDL-C and apoA-I levels, and inversely correlated with fasting blood glucose levels, blood pressure, BMI, and triglycerides. Multiple linear regression showed that HAE is significantly correlated with MetSyn (HAE: r2 = 0.57, P<0.0001), though MetSyn subjects on statins exhibited significantly higher HAE compared to subjects not on statins.
Conclusions: MetSyn has a significant negative impact on HDL remodelin/apoA-I exchange, as measured by HAE. HAE is a strong identifier of MetSyn status even after adjusting for individual components of MetSyn.
Author Disclosures: M.S. Borja: None. B. Hammerson: None. O.V. Savinova: None. G.C. Shearer: None. M.N. Oda: Ownership Interest; Modest; MNO is cofounder of Seer Biologics, Inc., which could stand to benefit from the research described here.. Other; Modest; MNO has filed a patent describing the assay through Children’s Hospital Oakland in 2011..
This research has received full or partial funding support from the American Heart Association.
- © 2015 by American Heart Association, Inc.