Abstract 612: Effect of Bariatric Surgery on HDL-Associated MicroRNAs
Background and aims: We have recently demonstrated that high-density lipoproteins (HDL) transport microRNAs (miRNAs) in the plasma. Here we aim to investigate how HDL-associated miRNAs are regulated in morbidly obese subjects and more importantly, how weight loss after bariatric surgery affected HDL-miRNAs in these subjects.
Methods: Plasma HDL were isolated from morbidly obese subjects (n=23) before, 6 months and 12 months after bariatric surgery by immunoprecipitation using goat anti-human apoA-I microbeads. We also isolated HDL from 12 normal subjects using the same methods. miRNAs were extracted from the isolated HDL and miR-223, miR-24 and miR-126 levels were determined by TaqMan miRNA assays.
Results: miR-223, miR-24 and miR-126 were present on HDL from obese subjects before and after bariatric surgery. Before surgery, levels of miR-223, miR-24 and miR-126 on HDL from morbidly obese subjects were significantly higher than the levels on HDL from normal subjects. At 12 months after bariatric surgery, miR-223, miR-24 and miR-126 levels on HDL from obese subjects were increased by 2.9-fold, 2.3-fold and 2.7-fold (p<0.05 all), respectively, compared to before surgery.
Conclusion: HDL-associated miRNAs are differentially regulated in healthy versus morbidly obese subjects. miR-223, miR-24 and miR-126 are sensitive to weight loss induced by bariatric surgery.
Author Disclosures: F. Tabet: None. K.C. Vickers: None. L.F. Cuesta Torres: None. B. Ammori: None. R. Yadav: None. S. Hama: None. Y. Liu: None. J. Schofield: None. P. Barter: None. H. Soran: None. K. Rye: None.
- © 2014 by American Heart Association, Inc.