Abstract 497: Carotid Stiffness and Atheromasic Plaque Composition: Biomolecular Assessment
The different histopathology of atherosclerotic plaques may modify carotid stiffness. In this work we evaluated if a correlation exists between stiffness, type of plaques, inflammatory and apoptotic molecules and Osteoprotegerin (OPG) expression. 19 patients were divided in two groups depending on type of plaque (Mixed and Fibrocalcific) established through Doppler-ultrasound. Among inflammatory molecules we considered p-JNK ,p-ERK and NLRP3 levels by Western Blotting analysis whereas among apoptotic molecules we evaluated TNFα and TNFαIIR, FAS and FAS-L, Bcl-2, Casp8, ASK1, OPG and HMGB1 levels by Real Time PCR. We observed increased OPG protein levels in mixed plaques compared with fibrocalcific ones according to expectations, because those plaques start to calcificate, as well as increased levels of all markers of inflammation and apoptosis, confirming a predictive role of these molecules relative to plaque composition. Stiffness and the Augmentation Index does not result affected to plaque composition but may depend on reduced size of patients recruited.
Author Disclosures: A. Bitto: None. F. Galfo: None. C. Zito: None. G. De Caridi: None. F. Squadrito: None.
- © 2014 by American Heart Association, Inc.