Abstract 488: Remote Ischemic Preconditioning Attenuates Renal Ischemia-Reperfusion Injury in a Model of Thoracoabdominal Aortic Aneurysm Open Repair
Introduction: Temporary renal ischemia consists a significant factor that increases morbidity and mortality after thoracoabdominal aortic aneurysm (TAAA) open repair. Various measures have been described to protect against renal ischemia-reperfusion (IR) injury. Remote ischemic preconditioning (RIPC) has been considered a means of decreasing IR injury in various tissues. Aim of the study was to identify the effect of RIPC in reducing renal IR injury in an animal model of TAAA open repair.
Material and methods: An animal based experimental study was designed. Twenty male swine were divided in 4 groups: Sham (control) group, IR group, RIPC I group, RIPC II group. Animals underwent a 30 minutes visceral ischemia after a concomitant subphrenic and infrarenal aortic clamping (IR group). In RIPC groups, two types of 15 minutes of lower body brief ischemia followed by 15 minutes of reperfusion, preceded the visceral ischemia (15 minutes ischemia and 15 minutes reperfusion in RIPC I group, and 3 cycles of 5 minutes ischemia followed by 5 minutes of reperfusion in RIPC II group). Elective renal vein blood was retrieved from the renal vein in specific time intervals of reperfusion, ranging from 30 minutes to 24 hours post-ischemia. Using ELISA techniques, serum was examined for various parameters indicating IR injury (MDA), inflammation and renal tissue damage (CRP, TNFa, Troponin I), as well as renal function (Urea, Creatinine, Cystatin C, NGAL).
Results: All examined parameters were found to be less impaired in animals of RIPC groups compared to IR group, at a significant statistical level.
Discussion: Blood data demonstrate a beneficial effect in reducing IR renal injury induced in a way similar to the one during a TAAA open repair, when RIPC has been preceded the IR injury.
Author Disclosures: A. Lazaris: None. D. Athanasiadis: None. A. Kapelouzou: None. M. Katsimpoulas: None. E. Balafas: None. S. Vasdekis: None. A. Kostakis: None. T. Liakakos: None.
- © 2014 by American Heart Association, Inc.