Abstract 323: Can Edaravone Protect From Kidney Damage Caused by Myonephropathic Metabolic Syndrome in Rats?
Introduction & Hypothesis: Free radicals have been implicated in myonephropathic metabolic syndrome (MNMS), which damages not only muscles but also kidneys. At ATVB 2012 & 2013, we reported the free radical scavenger, edaravone (Radicut®, Mitsubishi Tanabe Pharma Co., Japan) suppresses muscle injury by MNMS. In this study, we evaluated whether edaravone can suppress kidney damage caused by MNMS.
Methods: Lewis male rats (508 ±31 g, n = 10) were intraperitoneally injected with either 3.0 mg/kg of edaravone (edaravone group; n =4), or saline (MNMS group; n = 6). The MNMS models were induced, by clamping the bilateral common femoral arteries for 5 hours and then de-clamping. Five hours after de-clamping, the both kidneys were harvested and stained with hematoxylin & eosin. Normal kidneys were also harvested as control (n = 3). Kidney damage was evaluated by counting the number of cells in glomerulus (glomerular infiltration) and the density of residual tubular cells (enlargement of tubular cells).
Results: The number of cells in glomerulus was significantly increased in MNMS group than that in the control (77.2 ±3.7 vs. 49.6 ±2.8 cells/glomerulus, p <0.01). However, the number of cells in glomerulus was alleviated in edaravone group (62.2 ±2.4 cells/glomerulus, p <0.01). The density of residual tubular cells was significantly decreased in MNMS group than that in the control (555 ±24 vs. 957 ±44 cells/mm2, p <0.001). The density of residual tubular cells was maintained in edaravone group (654 ±38 cells/mm2, p =0.06).
Conclusions: MNMS was associated with significant glomerular infiltration and the enlargement of tubular cells. Edaravone may alleviate these kidney damages caused by MNMS.
Author Disclosures: M. Yamamura: Research Grant; Modest; Grant-in-Aid for Researchers, Hyogo College of Medicine 2012 & 2013. M. Mitsuno: None. H. Tanaka: None. M. Ryomoto: None. S. Fukui: None. Y. Miyamoto: None.
- © 2014 by American Heart Association, Inc.