Abstract 315: Surgical Pen Dye Inhibits Cell Migration From Saphenous Vein Explants
Markers containing dyes such as crystal violet (CAS 548-62-9) are routinely used on the adventitia of vein bypass grafts to avoid twisting during placement. Since little is known about how these dyes affect vein graft healing and function, we determined the effect of crystal violet on cell migration, a primary response to injury after grafting. Human saphenous veins were split into adventitia and media after removal of endothelium. Cell migration from 2.5 square mm explants in DMEM with 20% fetal bovine serum was measured daily for 8 days as either 1) % migration positive explants (≥ 1 cell/explant), which only measures migration, or 2) the number of cells/explant, which measures a combination of migration and proliferation. Dye was extracted from explants with ethanol and quantified by spectrophotometry. Cell migration by both methods was significantly less from blue, compared to non-blue, adventitial explants (P<.05, mean±SEM, N=11 veins with 15-30 explants/condition; Figures A and B). No medial explants were visibly blue, and there was no significant difference in migration of cells from medial explants of blue and non-blue sections of vein (data not shown). Blue adventitial explants had 65.9±8.0 ng dye/explant compared to 2.1±1.3 for non-blue explants (mean±SEM, N=7-11 veins). Dye applied in vitro to either adventitial or medial explants dose-dependently inhibited migration (IC50=21.5 ng/explant).
Conclusion: Crystal violet is toxic and inhibits venous adventitial cell migration; alternative methods should be considered for marking vein grafts.
Author Disclosures: S. Kikuchi: None. R.D. Kenagy: None. A.W. Clowes: None.
- © 2014 by American Heart Association, Inc.