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Poster Abstract PresentationsSession Title: Poster Session II

Abstract 302: Generation of Patient-Specific Tissue-Engineered Blood Vessels From Nonintegrated Induced Pluripotent Stem Cells

Yongyu Wang, Jiang Hu, Jiao Jiao, Y Eugene Chen, Peter Ma, Bo Yang
Arteriosclerosis, Thrombosis, and Vascular Biology. 2014;34:A302
Yongyu Wang
Cardiac Surgery, Univ of Michigan, Ann Arbor, MI
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Jiang Hu
Biologic and Materials Sciences, Univ of Michigan, Ann Arbor, MI
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Jiao Jiao
Cardiac Surgery, Univ of Michigan, Ann Arbor, MI
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Y Eugene Chen
Cardiac Surgery, Univ of Michigan, Ann Arbor, MI
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Peter Ma
Biologic and Materials Sciences, Univ of Michigan, Ann Arbor, MI
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Bo Yang
Cardiac Surgery, Univ of Michigan, Ann Arbor, MI
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Abstract

Cell resourcing remains one of the major problems in the development of patient-specific TEBVs. The iPSC derived cardiovascular cells provide a promising source for TEBVs and cell therapy. The goal of this study is to construct TEBVs using patient-specific iPSCs.

Using a non-integrated episomal vector based reprogramming system, we generated human iPSCs from peripheral blood mononuclear cells (PBMCs) isolated from the blood harvested from aneurysm patients with bicuspid aortic valves and coronary artery bypass patients with normal aortas and aortic valves. These iPSCs showed the expression of pluripotent markers and could differentiate into 3 layers of cells in vitro. We directed the iPSCs differentiation into vascular smooth muscle cells (VSMCs) in vitro. We documented the expression of VSMC markers, including smooth muscle α-actin (α-SMA), calponin and SM22α by quantitative real-time-PCR, immunocytochemistry, as well as Western-blotting. Furthermore, we confirmed that the iPSC-derived VSMCs could contract in response to carbachol, a muscarinic agonist. The treatment of iPSC-derived VSMCs with IL-1β, an atherogenic cytokine, changed MMPs (matrix metallopeptidases) activity. Finally, we found that iPSC-derived VSMCs could survive well and secrete collagen when transferred and cultured on PLLA scaffolds in vitro, suggesting a great cell sourcing for tissue-engineered blood vessels.

Our data suggests that by using non-integrated iPSCs, we could efficiently differentiate patient-specific iPSCs into functional VSMCs, which may provide great cell sourcing for patient-specific TEBVs.

Key Words:
  • tissue-engineered blood vessels (TEBVs)
  • induced pluripotent cells
  • vascular smooth muscle cells
  • Author Disclosures: Y. Wang: None. J. Hu: None. J. Jiao: None. Y. Chen: None. P. Ma: None. B. Yang: None.

  • © 2014 by American Heart Association, Inc.
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Arteriosclerosis, Thrombosis, and Vascular Biology
May 2014, Volume 34, Issue Suppl 1
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    Abstract 302: Generation of Patient-Specific Tissue-Engineered Blood Vessels From Nonintegrated Induced Pluripotent Stem Cells
    Yongyu Wang, Jiang Hu, Jiao Jiao, Y Eugene Chen, Peter Ma and Bo Yang
    Arteriosclerosis, Thrombosis, and Vascular Biology. 2014;34:A302, originally published September 3, 2014

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    Abstract 302: Generation of Patient-Specific Tissue-Engineered Blood Vessels From Nonintegrated Induced Pluripotent Stem Cells
    Yongyu Wang, Jiang Hu, Jiao Jiao, Y Eugene Chen, Peter Ma and Bo Yang
    Arteriosclerosis, Thrombosis, and Vascular Biology. 2014;34:A302, originally published September 3, 2014
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