Abstract 270: Reduction of Mitochondrial Antioxidant Levels Augments Osteogenic Signaling Independent of Changes in Vasomotor Function in Aorta from Hypercholesterolemic Mice
Mitochondrial antioxidant genes are major regulators of oxidative stress in the vasculature. Previous data has shown that reducing antioxidant capacity accelerates atherosclerotic plaque size, however, it is unclear whether reducing mitochondrial antioxidant capacity alters plaque composition. Therefore, we tested the hypothesis that reductions of mitochondrial antioxidant capacity will increase osteogenic markers and intimal plaque calcification in aorta from hypercholesterolemic mice. We used Ldlr-/-ApoB100/100 (LA) mice that were either wild-type (LA-MnSOD+/+) or heterozygous (LA-MnSOD+/-) for manganese superoxide dismutase that were fed a Western Diet (TD88137) for 3 or 6 months. Changes in changes gene expression were assessed in aortic arch using qRT-PCR, plaque calcium levels were examined using histological evaluation from aortic cryosections, and endothelial function was measured using isolated organ bath chambers (ex vivo). While expression of SP7 (an osteogenic transcription factor) was unchanged between LA-MnSOD+/+(1.0±0.6) and LA-MnSOD+/- (0.95±0.3) at 3 months, SP7 levels were dramatically increased in LA-MnSOD+/+ (8.0±2.8) at 6 months and increased further in LA-MnSOD+/+ mice (17.5±5.9; p<0.05). Alizarin Red staining for calcium showed increased calcium burden in plaques of 6 month LA-MnSOD+/- compared to LA-MnSOD+/+ (3636±669 pixels versus 649±206 pixels; p<0.05). Vasomotor function in aorta was unchanged between LA-MnSOD+/+ and LA-MnSOD+/- mice, suggesting that the transcriptional and functional changes related to calcification in LA-MnSOD+/- mice occur independently of nitric oxide signaling. In conclusion, our data strongly support our working hypothesis that reductions in mitochondrial antioxidant capacity significantly accelerate osteogenic calcification in aorta from hypercholesterolemic mice, and suggest that targeting mitochondrial oxidative stress may be a viable therapeutic strategy to slow vascular calcification.
Author Disclosures: C.M. Roos: None. M.A. Hagler: None. B. Zhang: None. A. Arghami: None. J.D. Miller: None.
- © 2014 by American Heart Association, Inc.