Abstract 188: Differentiation of Preexisting Cardiosphere-Derived Stem Cells Was Modulated by Telocyte Niche
Background: Cardiosphere-derived stem cells (CSCs) were found to be unusual pre-existing cells, comparing with other kinds of stem cells, showing the greatest cardiomyocytes differentiation potency. This study is to explore whether the microenvironment provided by telocytes can improve differentiation efficiency of CSCs.
Methods and Results: The CSCs and telocytes were isolated from myocardium of SD rats. The experiment was divided into control group, T-group (CSCs co-culture with adult rat telocytes), N-group (CSCs co-culture with neonatal rat caridomyocytes). Q-PCR was used to check the change of stem cell markers and cardiomyocyte markers. Immunofluorescence was used to detect the expression of cTnI protein in each group. The expression of Isl-1 in T-group was higher than other groups at 3 days, Nanong was higher at 5d, Gata4 and cTnI were higher at 9d. There are almost no expressions of cTnI protein in control group and N-group, but higher expressions of cTnI protein in T-group. The data of PCR-array showed that the most biomarkers of stem cells increased when CSCs co-culture with telocytes for 3 days, but the most biomarkers of cardiac progenitor cells increased when CSCs co-culture with telocytes for over 9 days. Finally, the CSCs induced by telocytes (iCSCs) were injected into borders of cardiac scar tissue 1 wk after experimental infarction. The results showed a nearly normal ejection fraction (EF) in iCSCs-treated rats but a lower EF in all PBS-treated animals.
Conclusion: There are bilateral regulations of the microenvironment provided by telocytes which not only maintain the stemness of CSCs at the early stage, but also improve the differentiation efficiency of CSCs at the late stage. Telocyte is an important chaperone of CSCs, and helps for repairing damaged heart muscle. This work was supported by NSFC (81330007) and 973 program of China (2012CB526600).
Author Disclosures: X. Yu: None. Y. Huang: None. Y. Pan: None. X. Li: None. Q. Lin: None. Z. Shan: None. C. Deng: None. M. Yang: None. Q. Geng: None. S. Lin: None.
- © 2014 by American Heart Association, Inc.