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Poster Abstract PresentationsSession Title: Poster Session I

Abstract 113: Screening of MicroRNAs Expressed in Isolated Cells of Human Abdominal Aortic Aneurysm for the Identification of Potential Biomarkers

Spear Rafaelle, Ludovic Boytard, Renaud Blervaque, David Hot, Jonathan Vanhoutte, Bart Staels, Maggy Chwastyniak, Philippe Amouyel, Stephan Haulon, Florence Pinet
Arteriosclerosis, Thrombosis, and Vascular Biology. 2014;34:A113
Spear Rafaelle
UMR-744, Institut Pasteur de Lille, Lille, France
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Ludovic Boytard
UMR-744, Institut Pasteur de Lille, Lille, France
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Renaud Blervaque
UMR-1019, Institut Pasteur de Lille, Lille, France
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David Hot
UMR-1019, Institut Pasteur de Lille, Lille, France
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Jonathan Vanhoutte
UMR-1011, Institut Pasteur de Lille, Lille, France
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Bart Staels
UMR-1011, Institut Pasteur de Lille, Lille, France
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Maggy Chwastyniak
UMR-744, Institut Pasteur de Lille, Lille, France
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Philippe Amouyel
UMR-744, Institut Pasteur de Lille, Lille, France
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Stephan Haulon
UMR-1008, CHRU de Lille, Lille, France
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Florence Pinet
UMR-744, Institut Pasteur de Lille, Lille, France
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Abstract

Abdominal aortic aneurysm (AAA) is a vascular asymptomatic disease that is one of the leading causes of death in developed countries. Identifying biomarkers for AAA that can be detected easily in blood, before rupture could be useful.

The aim of this study was to investigate the potentiality of miRNAS as biomarkers of AAA by performing a profiling of miRNAs expressed in major cells present in the human AAA tissue.

The inflammatory cells as macrophages M1 and M2 and smooth muscle cells (SMC) were located by immunohistochemistry in 20 human AAA biopsies, showing a specific distribution towards the aneurysmal aortic wall. The cells were isolated by laser microdissection (LMD) from 20 human AAA biopsies obtained during surgical repair and control SMC from 14 healthy aortic biopsies harvested during organs multiretrieval. RNA extracted from 2 samples of each LMD isolated cells was screened on human miRNAs microarray. MiRNAs were selected with at least a 2-fold change and a detection value threshold corresponding to the value of miR-29b, described in experimental AAA models.

Out of the 850 human miRNAs tested for each sample, 408 were found to be present in AAA. Thirty miRNAs were common to each tested cells. Fifty-three miRNAs were found in SMC, of which 12 were specific to AAA compared to control aortas; 86 miRNAs were found in macrophages, of which 11 were specific to M1 macrophages, 37 to M2 macrophages and 38 common to both subtypes. Ten miRNAs were selected to be validated by quantitative RT-PCR in LMD isolated healthy SMC and aneurysmal SMC, M1 and M2 macrophages. We validated 4 miRNAs to be overexpressed in M1 macrophages and 1 overexpressed in M2 macrophages. Two miRs were validated to be less expressed in aneurysmal SMC compared to SMC from normal aortas. MiR-29b expression was specifically down-regulated in aneurysaml SMC compared to normal SMC.

In conclusion, the analysis of isolated cells allows to discriminate the miRNAs specifically expressed in inflammatory and vascular cells in AAA and to determine other miRNAs than those described in experimental AAA models as potential biomarkers of AAA.

Key Words:
  • Abdominal aortic aneurysm
  • miRNA
  • human
  • Author Disclosures: S. Rafaelle: None. L. Boytard: None. R. Blervaque: None. D. Hot: None. J. Vanhoutte: None. B. Staels: None. M. Chwastyniak: None. P. Amouyel: None. S. Haulon: None. F. Pinet: None.

  • © 2014 by American Heart Association, Inc.
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May 2014, Volume 34, Issue Suppl 1
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    Abstract 113: Screening of MicroRNAs Expressed in Isolated Cells of Human Abdominal Aortic Aneurysm for the Identification of Potential Biomarkers
    Spear Rafaelle, Ludovic Boytard, Renaud Blervaque, David Hot, Jonathan Vanhoutte, Bart Staels, Maggy Chwastyniak, Philippe Amouyel, Stephan Haulon and Florence Pinet
    Arteriosclerosis, Thrombosis, and Vascular Biology. 2014;34:A113, originally published September 3, 2014

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    Abstract 113: Screening of MicroRNAs Expressed in Isolated Cells of Human Abdominal Aortic Aneurysm for the Identification of Potential Biomarkers
    Spear Rafaelle, Ludovic Boytard, Renaud Blervaque, David Hot, Jonathan Vanhoutte, Bart Staels, Maggy Chwastyniak, Philippe Amouyel, Stephan Haulon and Florence Pinet
    Arteriosclerosis, Thrombosis, and Vascular Biology. 2014;34:A113, originally published September 3, 2014
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