Abstract 53: Noninvasive Detection of Inflammation of Murine Stasis Deep Venous Thrombosis (DVT) Using Integrated 18F-FDG PET/CT
Background Noninvasive imaging of inflammation in DVT could offer new insights into the resolution of deep venous thrombosis (DVT) in living subjects. While a few clinical studies have reported incidental uptake of 18F-FDG PET in a subgroup of DVTs, limited information exists regarding the cellular mechanisms and time dependence of FDG uptake in DVT. Here we investigated whether FDG-PET could enhance murine stasis-induced DVT in acute to subacute DVT, and assessed the inflammatory cell infiltrate within DVT.
Methods C57BL/6 mice (n=6) underwent ligation of the jugular vein to induce stasis DVT. At DVT timepoints from day 2 to day 7, 18F-FDG PET (25-35 μCi/g) and contrast CT venography was performed. After PET/CT imaging, animals were sacrificed and veins were resected. Gamma-counting and histological analyses of thrombus neutrophils and macrophages were then performed.
Results PET/CT images demonstrated the FDG signal accumulated in thrombosed jugular veins in acute DVT, and significantly more than in contra-lateral sham veins (SUV = 1.27±0.11 vs. 0.79±0.069, SUVmax = 1.45±0.090 vs. 1.02±0.056, p<0.05, respectively). The %IDGT was significantly higher in thrombosed veins (7.21±0.17% vs. 1.54±0.47% sham vein, p<0.05). The % positive area of neutrophil in thrombus was 25 times higher than macrophages (14.1±2.9% vs. 0.58±0.091%, p<0.0001) in earlier-stage (day2-4) DVT.
Conclusions Noninvasive FDG-PET/CT can visualize inflammation in experimental DVT. Thrombus neutrophils appear to be the cellular source underlying accumulated FDG signal in earlier-stage DVT. FDG-PET may enable assessment of thrombus age and provide new insights into the resolution of DVT.
- © 2013 by American Heart Association, Inc.