Abstract 31: Vascular Repair by Adipose Stromal Fraction (SVF) cells in a Murine Model of Small Vessel Inflammation
The relatively few therapeutic options for treating small vessel disease creates a significant clinical challenge. The stromal vascular fraction (SVF) derived from adipose tissue is a rich source of regenerative cells shown to have anti-inflammatory and vascular reparative capabilities. We explored the ability of these SVF cells to reverse vascular insult in a murine model of artery inflammation induced by the placement of a 2 mm long polyethylene cuff around the left femoral artery in male wildtype FVB/n mice. One week after cuffing, freshly isolated SVF cells harvested from syngeneic FVB/nTg(CAG-luc,-GFP) mice ubiquitously and constitutively expressing the firefly luciferase and green fluorescent protein genes were injected via a tail vein. Sham mice, which underwent femoral exposure without receiving a cuff, were treated identically. Bioluminescence confirmed delivery and the persistent presence of SVF cells. Histological analysis of vessels indicated cuffed femoral arteries were surrounded by granulation tissue and exhibited intimal lesions associated with significant medial inward remodeling. In contrast, intravenous delivery of SVF cells after lesion formation, regardless of the dose, lessened intimal lesions, reversed medial remodeling, and reduced perivascular fibrosis. Immunostaining for luciferase identified SVF cells throughout the vessel wall and the surrounding extra-vascular tissue that were also positive for markers of M1 and M2 macrophages. In conclusion, intravenous delivery of freshly isolated adipose SVF cells reversed the vascular insult and peri-vascular granulation tissue due to inflammation.
- © 2013 by American Heart Association, Inc.