Abstract 288: Differences in Proliferative and Migratory Capability of Human Myoblasts Isolated From CLI and Control Patients
Objectives Patients with critical limb ischemia (CLI) have attributable myopathy that impairs their functional ability as well as dampening the positive effect of successful revascularisation outcomes. Adult skeletal muscle retains the potential to repair following different injury patterns. We aim to investigate the potential differences in proliferation and migration capability of human myoblasts with CLI and asymptomatic control patients.
Methodology Gastrocnemius muscle biopsies were obtained from patients with critical limb ischaemia and control samples were obtained from non-ischaemic patients. Human myoblasts were isolated and cultured from each sample and stained with the myoblast marker desmin. Confirmed myoblast cultures (<5% alternative cell type contamination) were then used to investigate the proliferative capability (MTT assay) as well as migratory potential (scratch-wound assay) under both ischaemic and normoxic conditions of each group.
Results Myoblasts isolated from CLI patients demonstrated greater proliferative ability in comparison to control samples. However, control samples revealed greater capacity to heal scratch wounds made in a monolayer of myoblasts in both normoxia and hypoxic conditions.
Conclusion Isolation of human myoblasts, especially from disease groups provides a useful platform to perform in vitro analysis to better understand and characterise pathology. CLI myoblasts exhibited enhanced proliferative but diminished migratory potential in comparison to control myoblasts.
- © 2013 by American Heart Association, Inc.