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Poster Abstract Presentations

Abstract 174: miR30c Regulates Plasminogen Activator Inhibitor-1 In Platelet

Mao Luo, Qin Wan, Fei Liu, Rong Li, Jiyi Xia, Meiping Ren, Ni Chen, Tammy Strawn, William P Fay, Jianbo Wu
Arteriosclerosis, Thrombosis, and Vascular Biology. 2013;33:A174
Mao Luo
Drug Discovery Rsch Cntr, Luzhou Med College, Luzhou, China
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Qin Wan
Dept of Endocriology, Luzhou Med College, Luzhou, China
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Fei Liu
Dept of Endocriology, Luzhou Med College, Luzhou, China
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Rong Li
Drug Discovery Rsch Cntr, Luzhou Med College, Luzhou, China
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Jiyi Xia
Drug Discovery Rsch Cntr, Luzhou Med College, Luzhou, China
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Meiping Ren
Drug Discovery Rsch Cntr, Luzhou Med College, Luzhou, China
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Ni Chen
Drug Discovery Rsch Cntr, Luzhou Med College, Luzhou, China
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Tammy Strawn
Internal Medicine, Univ of Missouri, Columbia, MO
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William P Fay
Internal Medicine, Univ of Missouri, Columbia, MO
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Jianbo Wu
Drug Discovery Rsch Cntr, Luzhou Med College, Luzhou, China
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Abstract

Objective. PAI-1 mRNA and protein have been detected in human platelets. Recently some miRNAs have been found in human platelets, which are involved in the regulation of genes and the protein synthesis. However, little is known about the physiological roles of individual miRNAs in platelets. In this study, we investigated whether miR30c can regulate platelet-derived plasminogen activator inhibitor-1(PAI-1).

Methods and Results. Expression of miR-30c, PAI-1, miR-21 and its targeted gene TIMP1 were found in healthy human leukocyte-depleted platelets (LDPs) by real time PCR. In luciferase reporter gene assay, miR-30c targets the 3’ untranslated region (3’ UTR) of PAI-1 mRNA through a miR-30c binding site. Transfection of miR-30c mimic into MEG-01, a megakaryoblastic cell line, significantly reduced PAI-1 protein level compared with negative control. Inhibition of miR-30c by transfecting miR-30c inhibitor significantly increased PAI-1 protein level. Furthermore, miR-21 expression was significantly down-regulated after transfecting with miR-30c mimic in PAI-1-/- mice LDPs, conversely, the expression of its target gene TIMP1 was significantly up-regulated after transfecting with miR-30c mimic in PAI-1-/- mice LDPs.

Conclusion. These results provide a novel regulatory mechanism of miR30c- regulated PAI-1 protein through its influence on the downstream miR21 and its target gene TIMP1 expression in platelet, suggesting that miR-30c might be a potential new strategy for anti-thrombosis.

  • platelet
  • microRNA
  • PAI-1
  • © 2013 by American Heart Association, Inc.
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April 2018, Volume 38, Issue 4
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    Abstract 174: miR30c Regulates Plasminogen Activator Inhibitor-1 In Platelet
    Mao Luo, Qin Wan, Fei Liu, Rong Li, Jiyi Xia, Meiping Ren, Ni Chen, Tammy Strawn, William P Fay and Jianbo Wu
    Arteriosclerosis, Thrombosis, and Vascular Biology. 2013;33:A174, originally published October 20, 2015

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    Abstract 174: miR30c Regulates Plasminogen Activator Inhibitor-1 In Platelet
    Mao Luo, Qin Wan, Fei Liu, Rong Li, Jiyi Xia, Meiping Ren, Ni Chen, Tammy Strawn, William P Fay and Jianbo Wu
    Arteriosclerosis, Thrombosis, and Vascular Biology. 2013;33:A174, originally published October 20, 2015
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