Abstract 164: Action of Nicotinic Acid on Adiponectin, Leptin and Plasminogen Activator Inhibitor 1 Production and Expression In 3T3-L1 Adipocytes Under Oxygen Deprivation.
Obesity has been considered one of the major contributors to the current global mortality. Recent studies suggest the dysregulation production of adipokines is partly responsible for obesity linked metabolic disorders.
The aim of this study was to investigate the effect of nicotinic acid (NA) on adipokines production and expression in 3T3-L1 adipocytes before and after hypoxia.
The mature adipocytes were pretreated with NA for 24h and induced by hypoxia for 4, 8 and 12h. Levels of adipokines in medium and total hypoxia inducible factor-1 alpha (HIF-1α) were quantified using immunoassay. Adipokines expression was analyzed by real time PCR.
Adipocytes treated with NA had lower leptin production in comparison to spontaneous production and an increase on leptin and Plasminogen Activator Inhibitor 1 (PAI-1) production associated with different periods of oxygen deprivation. It was observed a reduction of leptin levels in cells pretreated with NA compared to cells submitted to hypoxia. The same effect could be observed on PAI-1 production.
NA increased the production of adiponectin comparing to spontaneous production and cells pretreated witch NA increased adiponectin production during hypoxia.
Adipocytes were submitted to periods of oxygen deprivation had raised HIF-1α production comparing to spontaneous production and NA treatment. It also observed a reduction of HIF-1α in cells pretreated with NA and submitted to hypoxia.
Real time PCR showed a raise of adiponectin mRNA and a decreased PAI-1 in adipocytes treated with NA in comparison to spontaneous expression, but didn’t exert effect on spontaneous leptin.
Hypoxia, in all periods increased the leptin and PAI-1 expression and decreased adiponectin expression. In adipocytes pre-treated with NA blunted the production of leptin in spontaneous conditions and under hypoxia. It also increased PAI-1 production and expression. Adiponectin production and expression raised under NA treatment. The translation of such“in vitro” nicotinic acid benefits to the clinical setting should be object of future studies.
- © 2013 by American Heart Association, Inc.