Abstract 136: Quercetin Intake With Exercise Modulate Adipose Response in Mice Fed Atherogenic Diet
Adipose tissue is shown to influence atherosclerotic plaque formation and contribute to cardiovascular disease. Recent studies have focused on the potential role of adipose tissue physiopathology on several metabolic diseases.
Aims and Objectives In the current study we hypothesized that the combined action of quercetin and exercise will promote the releases of adipose anti-atherogenic metabolites and contribute to the reduction of the plaque formation.
Study Design C57BL6 LDL -/- mice fed atherogenic diet (40) was divided into four groups (10 each). These groups are as follows: Control mice, left untreated; control quercetin group, orally supplied with 100 μg/day of quercetin without exercising; exercise group without quercetin, and exercise group with quercetin supplements. The exercise groups were run on a treadmill for 30 minutes, 15m/m/ 5 days/week for 30 days. All animals were on atherogenic diet. At the end of the month of treatment, mice were sacrificed, and adipose tissue gene expressions for selected genes were analyzed.
Results and Conclusion We previously reported that mice on quercetin and exercise have significant (P<0.001) drop in body weigh accompanied with a 78% drop in the atherosclerotic plaque development in exercising groups. In the current abstract we investigated whether the drop in weight is mediated through an adipose tissue based mechanism. The adipose gene expression studies have shown significant (P<0.0001) up regulation of PPARg in exercised mice compared to the control and quercetin fed group, and PPARg also increased with exercise and quercetin intake although these changes were not significant. Adiponectin on the other hand elevated with exercise or quercetin intake alone and there was no appreciable changes with the combination of exercise and quercetin intake compared to control. Exercise; quercetin or the combination increased SREBP-1; however, interestingly quercetin intake with exercise significantly increased leptin up regulation. Circulating leptin levels are known to correlate with white adipose tissue mass. This finding brings an amazing perspective to the role of antioxidants taken with exercise which need further investigation to establish its mechanism(s) that modulate adipose metabolism.
- © 2013 by American Heart Association, Inc.