Abstract 545: Redox-Sensitive Regulation of Bone Marrow Hematopoietic Progenitor Cells
Bone marrow failure is a group of hematopoietic stem cell disorders that affect one or more lineages of blood cells. Hematopoietic progenitor cells are highly sensitive to reactive oxygen species (ROS)-induced oxidative stress. ROS impair the self-renewal of hematopoietic progenitor cells in the bone marrow and cause bone marrow failure. Glutaredoxin (Grx) is an antioxidant enzyme important for many cellular processes such as resistance against oxidative stress, DNA synthesis, sulfur assimilation, apoptosis, and cellular differentiation. However, the role of Grx in hematopoietic progenitor cells remains unknown.
We study the role of Grx in hematopoietic progenitor cells using Grx KO mice as the model system. Bone marrow cells were isolated from 9-10 weeks old wild type and Grx KO mice and the ratio of KSL cells, total blood count and p38 activation was studied. Our results showed no significant difference on the ratio of KSL cells between wild type and Grx KO mice in basal condition. There is also no significant difference in the numbers of red blood cells, lymphocytes, white blood cells and platelet in basal condition between wild type mice and Grx KO. Interestingly, upon hydrogen peroxide treatment, there is higher level of p38 activation in Grx KO mice compared to wild type mice. Our results suggest that Grx is involved in redox-sensitive regulation of hematopoietic progenitor cells. Grx may play a regulatory role in the prevention of bone marrow failure.
- © 2012 by American Heart Association, Inc.