Abstract 454: Impact of Uremia on Human Adipose Phenotype
Background: Recognition of adipose-related signaling participation in vascular processes is increasing, though direct interrogation of clinically relevant human adipose has been sparse. Few scenarios rival uremia for health impact. To advance understanding of the spectrum/determinants of human adipose biology, we compared fresh extremity adipose from patients with/without uremia.
Methods: Subcutaneous adipose and clinical data was collected from patients undergoing open elective surgery. Protein was isolated, and mediator levels determined via multiplex assays.
Results: In the 71 patient cohort, mean age=63.4y; 63.3% had diabetes, 49.2% had hyperlipidemia and 53.5% had coronary disease. Compared to non-uremic patients, uremic patients had 1/10th the levels of leptin, 1/3rd the levels of adiponectin, and 3-fold higher levels of resistin (Table 1). Females had 6-fold higher leptin, 1.5-fold higher adiponectin and 2-fold higher TNF but equivalent resistin. There were no differences in mediator levels when stratified by age. In both the obese/non-obese strata, we observed a concordant pattern of association (magnitude/significance) of uremia and leptin/adiponectin/resistin. No differentials in other mediators emerged upon BMI stratification. Multiple regression analysis for leptin/adiponectin/resistin (with age/gender/uremia as independent variables) showed uremia as the highest independent predictor of all three mediators, followed by gender, whereas age had no effect.
Conclusions: Based on direct biopsy of living human adipose, uremia is associated with adiposopathy, including perturbations in the adipose derived hormones leptin, adiponectin, and resistin. Compared with other parameters including BMI, uremia overall dominated in determining adipose phenotype.
- © 2012 by American Heart Association, Inc.