Skip to main content
  • American Heart Association
  • Science Volunteer
  • Warning Signs
  • Advanced Search
  • Donate

  • Home
  • About this Journal
    • Editorial Board
    • Meet the Editors
    • ATVB Journal History
    • General Statistics
  • All Issues
  • Subjects
    • All Subjects
    • Arrhythmia and Electrophysiology
    • Basic, Translational, and Clinical Research
    • Critical Care and Resuscitation
    • Epidemiology, Lifestyle, and Prevention
    • Genetics
    • Heart Failure and Cardiac Disease
    • Hypertension
    • Imaging and Diagnostic Testing
    • Intervention, Surgery, Transplantation
    • Quality and Outcomes
    • Stroke
    • Vascular Disease
  • Browse Features
    • Cover Art Award
    • ATVB Early Career Award
    • ATVB in Focus
    • Recent Brief Reviews of ATVB
    • Lecture Series
    • Collections
    • Recent Highlights of ATVB
    • Commentaries
    • Browse Abstracts
    • Insight into ATVB Authors
  • Resources
    • Instructions for Authors
    • Online Submission/Peer Review Site
    • Council on ATVB
    • Permissions and Rights Q&A
    • AHA Guidelines and Statements
    • Customer Service and Ordering Information
    • Author Reprints
    • International Users
    • AHA Newsroom
  • AHA Journals
    • AHA Journals Home
    • Arteriosclerosis, Thrombosis, and Vascular Biology (ATVB)
    • Circulation
    • → Circ: Arrhythmia and Electrophysiology
    • → Circ: Genomic and Precision Medicine
    • → Circ: Cardiovascular Imaging
    • → Circ: Cardiovascular Interventions
    • → Circ: Cardiovascular Quality & Outcomes
    • → Circ: Heart Failure
    • Circulation Research
    • Hypertension
    • Stroke
    • Journal of the American Heart Association
  • Facebook
  • LinkedIn
  • Twitter

  • My alerts
  • Sign In
  • Join

  • Advanced search

Header Publisher Menu

  • American Heart Association
  • Science Volunteer
  • Warning Signs
  • Advanced Search
  • Donate

Arteriosclerosis, Thrombosis, and Vascular Biology

  • My alerts
  • Sign In
  • Join

  • Facebook
  • LinkedIn
  • Twitter
  • Home
  • About this Journal
    • Editorial Board
    • Meet the Editors
    • ATVB Journal History
    • General Statistics
  • All Issues
  • Subjects
    • All Subjects
    • Arrhythmia and Electrophysiology
    • Basic, Translational, and Clinical Research
    • Critical Care and Resuscitation
    • Epidemiology, Lifestyle, and Prevention
    • Genetics
    • Heart Failure and Cardiac Disease
    • Hypertension
    • Imaging and Diagnostic Testing
    • Intervention, Surgery, Transplantation
    • Quality and Outcomes
    • Stroke
    • Vascular Disease
  • Browse Features
    • Cover Art Award
    • ATVB Early Career Award
    • ATVB in Focus
    • Recent Brief Reviews of ATVB
    • Lecture Series
    • Collections
    • Recent Highlights of ATVB
    • Commentaries
    • Browse Abstracts
    • Insight into ATVB Authors
  • Resources
    • Instructions for Authors
    • Online Submission/Peer Review Site
    • Council on ATVB
    • Permissions and Rights Q&A
    • AHA Guidelines and Statements
    • Customer Service and Ordering Information
    • Author Reprints
    • International Users
    • AHA Newsroom
  • AHA Journals
    • AHA Journals Home
    • Arteriosclerosis, Thrombosis, and Vascular Biology (ATVB)
    • Circulation
    • → Circ: Arrhythmia and Electrophysiology
    • → Circ: Genomic and Precision Medicine
    • → Circ: Cardiovascular Imaging
    • → Circ: Cardiovascular Interventions
    • → Circ: Cardiovascular Quality & Outcomes
    • → Circ: Heart Failure
    • Circulation Research
    • Hypertension
    • Stroke
    • Journal of the American Heart Association
Poster Abstract PresentationsSession Title: Poster Session II

Abstract 355: β-Adrenergic Stimulation Impact on Cerebrovascular Contractility Through Actin Cytoskeleton Disorganization in Rabbits

Nari Kim, Hyoung Kyu Kim, Hye-Jin Heo, Kyung Soo Ko, Byoung Doo Rhee, Jin Han
Arteriosclerosis, Thrombosis, and Vascular Biology. 2012;32:A355
Nari Kim
Cardiovascular and Metabolic Disease Cntr, Inje Univ, Busan, Korea, Republic of
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hyoung Kyu Kim
Cardiovascular and Metabolic Disease Cntr, Inje Univ, Busan, Korea, Republic of
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hye-Jin Heo
Cardiovascular and Metabolic Disease Cntr, Inje Univ, Busan, Korea, Republic of
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kyung Soo Ko
Cardiovascular and Metabolic Disease Cntr, Inje Univ, Busan, Korea, Republic of
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Byoung Doo Rhee
Cardiovascular and Metabolic Disease Cntr, Inje Univ, Busan, Korea, Republic of
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jin Han
Cardiovascular and Metabolic Disease Cntr, Inje Univ, Busan, Korea, Republic of
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics

Jump to

  • Article
  • Info & Metrics
  • eLetters
Loading

Abstract

Beta-adrenoceptor stimulations have been well-known to initiate cardiac hypertrophy accompanied by extracellular remodeling that predisposes heart failure and cardiac dysarrhythmia. However, less is known concerning cerebrovascular dysfunction induced by beta-adrenergic stimulation. We investigated whether cerebrovascular functions and related biochemical signal pathways were altered during beta-adrenergic stimulations in rabbits.

New Zealand white rabbits have been injected with isoproterenol (ISO, 0.3 mg/kg/day) for 7 days. We have studied the alteration of protein expression in cerebral arteries (CAs) using 2DE proteomics and western blot analysis and further analyzed those data using bioinformatics software. Reactive oxygen species generation and followed DNA damage were assessed deteriorative effect of ISO on CAs. Vasocontractility response to angiotensin II (Ang II) and related biochemical signal pathways were assessed to evaluate functional alteration of CAs.

Proteomic analysis revealed remarkably decreased expression of cytoskeleton organizing proteins, (e.g. actin related protein 1A and 2, α-actin, capping protein Z beta, and vimentin) and anti-oxidative stress proteins (e.g. heat shock protein 9A and stress-induced-phosphoprotein 1) in ISO-stimulated CAs. As a cause of dysregulation of actin cytoskeleton organization, we found decreased level of RhoA/ROCK1, major regulators of actin cytoskeleton organization. As functional consequences of proteomic alteration, we found the decreased transient Ca2+ efflux and contraction response to Ang II and high K+ in ISO-stimulated CAs.

We suggested that ISO stimulations reduced RhoA/ROCK activities and modified key actin based cytoskeletal and antioxidative proteins in CAs, which consequently lead to impair cerebrovascular contractility response to Ang II in rabbits.

  • beta-adrenergic stimulations
  • cerebrovascular dysfunction
  • actin cytoskeleton
  • oxidative stress
  • 2DE-proteomics
  • © 2012 by American Heart Association, Inc.
Back to top

Current Issue

Arteriosclerosis, Thrombosis, and Vascular Biology
May 2018, Volume 38, Issue 5
  • Table of Contents

Jump to

  • Article
  • Info & Metrics

Article Tools

  • Citation Tools
    Abstract 355: β-Adrenergic Stimulation Impact on Cerebrovascular Contractility Through Actin Cytoskeleton Disorganization in Rabbits
    Nari Kim, Hyoung Kyu Kim, Hye-Jin Heo, Kyung Soo Ko, Byoung Doo Rhee and Jin Han
    Arteriosclerosis, Thrombosis, and Vascular Biology. 2012;32:A355, originally published October 20, 2015

    Citation Manager Formats

    • BibTeX
    • Bookends
    • EasyBib
    • EndNote (tagged)
    • EndNote 8 (xml)
    • Medlars
    • Mendeley
    • Papers
    • RefWorks Tagged
    • Ref Manager
    • RIS
    • Zotero
  • Article Alerts
    Log in to Email Alerts with your email address.
  • Save to my folders

Share this Article

  • Email

    Thank you for your interest in spreading the word on Arteriosclerosis, Thrombosis, and Vascular Biology.

    NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

    Enter multiple addresses on separate lines or separate them with commas.
    Abstract 355: β-Adrenergic Stimulation Impact on Cerebrovascular Contractility Through Actin Cytoskeleton Disorganization in Rabbits
    (Your Name) has sent you a message from Arteriosclerosis, Thrombosis, and Vascular Biology
    (Your Name) thought you would like to see the Arteriosclerosis, Thrombosis, and Vascular Biology web site.
  • Share on Social Media
    Abstract 355: β-Adrenergic Stimulation Impact on Cerebrovascular Contractility Through Actin Cytoskeleton Disorganization in Rabbits
    Nari Kim, Hyoung Kyu Kim, Hye-Jin Heo, Kyung Soo Ko, Byoung Doo Rhee and Jin Han
    Arteriosclerosis, Thrombosis, and Vascular Biology. 2012;32:A355, originally published October 20, 2015
    del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo

Related Articles

Cited By...

Arteriosclerosis, Thrombosis, and Vascular Biology

  • About ATVB
  • AHA CME
  • Meeting Abstracts
  • Permissions
  • Email Alerts
  • Open Access Information
  • AHA Journals RSS
  • AHA Newsroom

Contact the Editorial Office:
email: atvb@atvb.org

Information for:
  • Advertisers
  • Subscribers
  • Subscriber Help
  • Institutions / Librarians
  • Institutional Subscriptions FAQ
  • International Users
American Heart Association Learn and Live
National Center
7272 Greenville Ave.
Dallas, TX 75231

Customer Service

  • 1-800-AHA-USA-1
  • 1-800-242-8721
  • Local Info
  • Contact Us

About Us

Our mission is to build healthier lives, free of cardiovascular diseases and stroke. That single purpose drives all we do. The need for our work is beyond question. Find Out More about the American Heart Association

  • Careers
  • SHOP
  • Latest Heart and Stroke News
  • AHA/ASA Media Newsroom

Our Sites

  • American Heart Association
  • American Stroke Association
  • For Professionals
  • More Sites

Take Action

  • Advocate
  • Donate
  • Planned Giving
  • Volunteer

Online Communities

  • AFib Support
  • Garden Community
  • Patient Support Network
  • Professional Online Network

Follow Us:

  • Follow Circulation on Twitter
  • Visit Circulation on Facebook
  • Follow Circulation on Google Plus
  • Follow Circulation on Instagram
  • Follow Circulation on Pinterest
  • Follow Circulation on YouTube
  • Rss Feeds
  • Privacy Policy
  • Copyright
  • Ethics Policy
  • Conflict of Interest Policy
  • Linking Policy
  • Diversity
  • Careers

©2018 American Heart Association, Inc. All rights reserved. Unauthorized use prohibited. The American Heart Association is a qualified 501(c)(3) tax-exempt organization.
*Red Dress™ DHHS, Go Red™ AHA; National Wear Red Day ® is a registered trademark.

  • PUTTING PATIENTS FIRST National Health Council Standards of Excellence Certification Program
  • BBB Accredited Charity
  • Comodo Secured