Abstract 110: Deficiency of Apolipoprotein F Protects Mice from Western Diet-Induced Atherosclerosis on an LDL Receptor Knockout Background
Apolipoprotein F (ApoF) is an acidic sialogycoprotein found on high density and low density lipoproteins. We have generated mice lacking the ApoF gene on a pure C57BL/6 background. These mice were crossed with Low Density Lipoprotein Receptor (LDLR) deficient mice to investigate a potential role for ApoF in atherogenesis. LDLR KO and ApoF/LDLR double knockout (DKO) mice were fed a western diet containing 0.21 % cholesterol 21 % fat (w/w). The aortas were removed and stained with Oil Red-O to quantify lesion area by en face analysis. After 16 weeks of feeding, male ApoF/LDLR DKO mice had a 36% reduction in lesional area relative to LDLR KO control animals (DKO 4.9 +/- 1.6% versus LDLR 7.7 +/- 2.1%; p <0.05). Likewise, after 17 weeks of western diet feeding, female ApoF/LDLR DKO mice showed a 38% reduction in lesional area (DKO: 4.9 +/- 1.5 versus LDLR: 7.8 +/- 1.0; p < 0.05). Plasma total cholesterol, HDL cholesterol, and triglycerides did not significantly differ by ApoF genotype on the western diet. The data suggest the ApoF locus may play an important role in atherosclerosis, likely through mechanisms not directly related to plasma cholesterol levels.
- © 2012 by American Heart Association, Inc.