In the Original Contribution “Deficiency of Interleukin-1 Receptor Antagonist Induces Aortic Valve Disease in BALB/c Mice” by Isoda et al, which appeared in the April 2010 issue of the journal (Arterioscler Thromb Vasc Biol. 2010;30:708–715; DOI: 10.1161/ATVBAHA.109.201749), the publisher omitted several important corrections from the final, published version:
Page 708, 2nd line of abstract: the word “degenerative” should be deleted, for the correct version to read: “However, the role of IL-1Ra in aortic valve stenosis (AS) remains poorly understood.”
Page 709, first line of “Echocardiography” section: the words “and heart” should be inserted between valve and function, for the correct version to read: “To examine the valve and heart function, we performed transthoracic echocardiography using a Sonos 550-U (Phillips) equipped with 12-MHz and 15-MHz imaging transducers.”
Page 709, 1st line of 2nd column: the words “(male; age, 40 weeks) should have been deleted, for the correct verstion to read: “After anesthetizing the mice with 2,2,2-tribromoethanol (250 mg/g intraperitoneal), we shaved their chests and attached ECG leads to each limb using needle electrodes.”
Page 709, 2nd line of Figure 1 legend, the word “degenerative” should have been deleted and “aortic valve disease” should be replaced with the abbreviation “AS,” for the correct version to read: “IL-1Ra−/− mice spontaneously had aortic valve disease independent of systolic blood pressure and cholesterol profiles.”
Page 711, 1st line of Table footnote, a hyphen should be inserted between “end” and “diastole” and “end” and “systole,” for the correct version to read: “ED indicates end-diastole; ES, end-systole …”
Page 712, 3rd line of second column, the word “Finally” should begin a new paragraph.
Page 712, 2nd line of “Discussion” section, the word “degenerative” should be deleted, for the correct version to read: “Our study demonstrates that IL-1Ra−/− mice on BALB/c background spontaneously developed aortic valve disease and inflammation.”
Page 713, last line of first column, the sentence beginning with “In contrast …” should be a new paragraph, in addition, the words “In contrast” should be replaced with the sentence “Almost all IL-1Ra−/− mice had aortic valve lesions develop, but about only 50% of IL-1Ra−/− mice had aortitis develop. There may be several reasons for the different rates between them. First…” The correct version should read: Almost all IL-1Ra−/− mice had aortic valve lesions develop, but about only 50% of IL-1Ra−/− mice had aortitis develop. There may be several reasons for the different rates between them. First, our preliminary data showed that although IL-1Ra−/− mice on a C57BL/6J background had mild aortic valve swelling, they did not have aortitis …”
Page 713, 4th line of second column, the word “In addition,” should be replaced with “Second,” for the correct version to read: “Second, the degree of inflammation differed among individual mice and several mice had aortitis …”
Page 713, 11th line of 2nd column, the sentence starting with “Histological analysis …” should start a new paragraph.
Page 713, Figure 5 legend, 1st line, the word “upper” should be deleted, 2nd line, the words “upper right” should be replaced with the word “middle” and the words “lower left” should be replaced with the word “right,” last line, the word “(left)” should be replaced with the word “(right)” for the correct version to read: “A, Plasma levels of IL-1β (left), TNF-α (middle), and IL-6 (right) in both IL-1Ra−/−and WT mice (n=6, **P<0.01). B, Macroscopic appearance of the aortic valve of IL-1Ra−/− (TNF+/+/Ra−/−; upper left) and double-gene-deficient (TNF−/−/Ra−/−; lower left; MT staining; bar, 200 μm). We analyzed mean aortic valve leaflet thickness and the mean thickness of WT mice at 12 weeks old was used as a reference against that of TNF+/+/Ra−/− and TNF−/−/Ra−/− mice (**P<0.01; n=6; right panel). C, Representative photomicrographs depict immunohistochemical staining for TNF- α in aortic valve of TNF+/+/IL-1Ra−/− (left) and TNF- α−/−/IL-1Ra−/− (right) mice (8 weeks old).”
Page 714, 7th line of first column, the word “inflammatory cells” should be inserted between “deficiency” and “may” for the correct version to read: “Because those findings suggested that IL-1Ra deficiency in inflammatory cells may promote AS, we performed BM cell transplantation.”
Page 714, conclusion section, the current conclusion section should be the last paragraph of the “Discussion” section, the following should have been inserted as the “Conclusion” section: “We show here that IL-1Ra−/− mice on the BALB/c background spontaneously develop aortic valve disease. We further suggest that IL-1Ra-deficiency in inflammatory cells induces aortic valve inflammation, and TNF-α participates importantly in the development of AS in IL-1Ra−/− mice.”
The online version of the article has been corrected.
The publisher sincerely regrets the errors.