Chylomicronemia Elicits Atherosclerosis in Mice—Brief Report
Objective— The risk of atherosclerosis in the setting of chylomicronemia has been a topic of debate. In this study, we examined susceptibility to atherosclerosis in Gpihbp1-deficient mice (Gpihbp1−/−), which manifest severe chylomicronemia as a result of defective lipolysis.
Methods and Results— Gpihbp1−/− mice on a chow diet have plasma triglyceride and cholesterol levels of 2812±209 and 319±27 mg/dL, respectively. Even though nearly all of the lipids were contained in large lipoproteins (50 to 135 nm), the mice developed progressive aortic atherosclerosis. In other experiments, we found that both Gpihbp1-deficient “apo-B48–only” mice and Gpihbp1-deficient “apo-B100–only” mice manifest severe chylomicronemia. Thus, GPIHBP1 is required for the processing of both apo-B48– and apo-B100–containing lipoproteins.
Conclusions— Chylomicronemia causes atherosclerosis in mice. Also, we found that GPIHBP1 is required for the lipolytic processing of both apo-B48– and apo-B100–containing lipoproteins.
Human and mouse studies have proven that high levels of cholesterol-rich remnants cause atherosclerosis, but the relevance of triglyceride-rich lipoproteins (TRLs) to atherogenesis remains controversial.1 In part, this controversy stems from the fact that elevated levels of TRLs and remnants often coexist.
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Humans with chylomicronemia have severe hypertriglyceridemia but low levels of cholesterol-rich remnants. Chylomicronemia patients are often assumed to be protected from atherosclerosis because experiments in rabbits had shown that very large lipoproteins cannot enter the arterial wall and were not particularly atherogenic.2 However, this assumption was recently challenged by the finding of atherosclerosis in 4 chylomicronemia patients with lipoprotein lipase (LPL) deficiency.3
Mice lacking GPIHBP1 (Gpihbp1−/−) have severe chylomicronemia, even on a low-fat chow diet, as a result of defective lipolysis.4 Very recently, chylomicronemia has been observed in a young man with a homozygous missense mutation in GPIHBP1.5 Thus far, no one has examined whether Gpihbp1−/− mice have an increased susceptibility to atherosclerotic lesions. In this study, we examined that issue.
We also tackled a second issue; we addressed whether GPIHBP1 is required for the lipolytic processing of both apo-B48–containing lipoproteins and apo-B100–containing lipoproteins.
Materials and Methods
Gpihbp1−/− mice (>90% C57BL/6)4 were housed in a barrier facility and fed a 4.5%-fat chow diet. We also bred “apo-B48–only” and “apo-B100–only” Gpihbp1−/− mice (Gpihbp1−/−Apob48/48, Gpihbp1−/−Apob100/100).6 All experiments were approved by the Animal Research Committee.
Plasma lipid levels were measured with enzymatic kits, and their distribution within lipoproteins was assessed by fast protein liquid chromatography (FPLC).6 Lipoprotein diameters were measured by laser light scattering.6 Western blots were performed as described.6
Sections of the aortic root were stained with Oil Red O, and lesions were quantified as described.6 Macrophages were stained with a monoclonal antibody against CD68 (Serotec; 1:100).
Gpihbp1−/− mice had lipemic plasma (Figure 1A), and nearly all of the plasma lipids were contained in large lipoproteins (as judged by FPLC; Figure 1B). Also, plasma apo-B48 levels in Gpihbp1−/− mice were increased (Figure 1C and 1D). Gpihbp1−/− mice had triglyceride and cholesterol levels of 2812±209 and 319±27 mg/dL, respectively (n=42; versus 35±5 and 90±6 mg/dL in littermate Gpihbp1+/+ mice, n=24; P<0.0001 for both; Figure 1E).
The fact that amphibians and birds have neither apo-B48 nor GPIHBP1 led Beigneux et al4 to speculate that GPIHBP1 might have arisen in mammals as a new protein specifically dedicated to the lipolytic processing of apo-B48–lipoproteins. They further hypothesized that GPIHBP1 might be unimportant for the processing of apo-B100–containing lipoproteins and that mice lacking both apo-B48 and GPIHBP1 might be normolipidemic. Although this hypothesis initially seemed attractive, it was incorrect. Gpihbp1−/−Apob+/+ mice and littermate Gpihbp1−/−Apob48/48 mice had very similar plasma lipid levels (Figure 1F). Similarly, Gpihbp1−/− Apob+/+ mice and littermate Gpihbp1−/−Apob100/100 mice had very similar plasma lipid levels (Figure 1G).
Most of the d<1.006 g/mL lipoproteins in Gpihbp1−/− mice were large (50 to 135 nm), overlapping little with the sizes of lipoproteins in Gpihbp1+/+ mice (Figure 1H). Lipoprotein sizes were similar in Gpihbp1−/−Apob+/+ mice and littermate Gpihbp1−/−Apob48/48 mice (Figure 1I). Also, lipoprotein sizes were similar in Gpihbp1−/−Apob+/+ mice and littermate Gpihbp1−/−Apob100/100 mice (Figure 1J).
Chow-fed Gpihbp1−/− mice developed lipid-rich atherosclerotic lesions in the aortic root and coronary arteries (Figure 2A and 2B). The lesions also stained strongly for macrophages (Figure 2C). The lesions were small at 11 to 12 months (median 500 μm2/section) but were much larger at 16 to 22 months (median 3000 μm2/section; Figure 2D). Lesions were evident in both males and females (3947; 3172 to 6071 [median; 33rd to 66th percentile] and 1680; 1000 to 4688 μm2/section, respectively, P=0.74).
GPIHBP1-deficient mice develop severe chylomicronemia as a result of defective lipolysis of triglyceride-rich lipoproteins.4 In this study, we address two timely and important issues. First, we show that mice with chylomicronemia develop spontaneous atherosclerosis, despite the fact that most of the lipids in these mice are found in large lipoproteins—lipoproteins that are often considered to be nonatherogenic.2 Thus, chylomicronemia leads to atherosclerotic lesions, even in mice fed a low-fat chow diet. These findings in mice add plausibility to the concept3 that chylomicronemia in humans could lead to increased susceptibility to atherosclerosis. Second, we show that the severe chylomicronemia in Gpihbp1−/− mice is not attributable to a selective defect in the processing of apo-B48–containing lipoproteins. Beigneux et al4 had hypothesized that GPIHBP1 might be a mammalian protein specifically dedicated to the processing of apo-B48–containing lipoproteins and further speculated that the processing of apo-B100–containing lipoproteins might not depend on GPIHBP1. This speculation is incorrect. The plasma lipid levels and lipoprotein sizes in Gpihbp1−/−Apob+/+ mice are very similar to those in littermate Gpihbp1−/−Apob100/100 mice (or littermate Gpihbp1−/−Apob48/48 mice). Thus, GPIHBP1 is required for the processing of both apo-B48–containing lipoproteins and apo-B100–containing lipoproteins.
Finding atherosclerosis in Gpihbp1−/− mice is consistent with a recent report of aortic lesions in Lpl−/− mice that had been rescued with an injection of an LPL adenovirus.7,8 The severity of the hypertriglyceridemia in Gpihbp1−/− mice and LPL adenovirus–rescued Lpl−/− mice is quite similar; however, Gpihbp1−/− mice appear to be the most reasonable choice for future research. Gpihbp1−/− mice can be bred in limitless numbers, whereas the production of adenovirus-rescued Lpl−/− mice is more challenging. Also, systemic infections with an adenovirus could cloud the interpretation of mouse atherosclerosis studies.
The lesions in Gpihbp1−/− mice are small and require longer to develop than those of Apoe−/− or Ldlr−/− mice.6 Nevertheless, these mice will be useful to the research community. With Gpihbp1−/− mice, it should be possible to determine whether the atherogenicity of TRLs depends on their cholesterol content. Also, this model opens the door to defining the impact of different dietary fatty acids (including dietary oxidized fatty acids) on the atherogenicity of TRLs.
Sources of Funding
This work was supported by grants HL094732-01 (to A.P.B.); T32HL69766 (to M.M.W.); Texas AgriLife 8738 (to R.L.W.); HL090553 and HL087228 (to S.G.Y.).
M.M.W. and L.Y. contributed equally to this study.
Received November 20, 2008; revision accepted September 24, 2009.
Nordestgaard BG, Zilversmit DB. Large lipoproteins are excluded from the arterial wall in diabetic cholesterol-fed rabbits. J Lipid Res. 1988; 29: 1491–1500.
Beigneux AP, Davies B, Gin P, Weinstein MM, Farber E, Qiao X, Peale P, Bunting S, Walzem RL, Wong JS, Blaner WS, Ding ZM, Melford K, Wongsiriroj N, Shu X, de Sauvage F, Ryan RO, Fong LG, Bensadoun A, Young SG. Glycosylphosphatidylinositol-anchored high density lipoprotein–binding protein 1 plays a critical role in the lipolytic processing of chylomicrons. Cell Metab. 2007; 5: 279–291.
Beigneux AP, Franssen R, Bensadoun A, Gin P, Melford K, Peter J, Walzem RL, Weinstein MM, Davies BS, Kuivenhoven JA, Kastelein JJ, Fong LG, Dallinga-Thie GM, Young SG. Chylomicronemia with a mutant GPIHBP1 (Q115P) that cannot bind lipoprotein lipase. Arterioscler Thromb Vasc Biol. 2009; 29: 956–962.
Zhang X, Qi R, Xian X, Yang F, Blackstein M, Deng X, Fan J, Ross C, Karasinska J, Hayden MR, Liu G. Spontaneous atherosclerosis in aged lipoprotein lipase deficient mice with severe hypertriglyceridemia on a normal chow diet. Circ Res. 2008; 102: 250–256.
Strauss JG, Frank S, Kratky D, Hammerle G, Hrzenjak A, Knipping G, von Eckardstein A, Kostner GM, Zechner R. Adenovirus-mediated rescue of lipoprotein lipase-deficient mice. Lipolysis of triglyceride-rich lipoproteins is essential for high density lipoprotein maturation in mice. J Biol Chem. 2001; 276: 36083–36090.
Lo CM, Nordskog BK, Nauli AM, Zheng S, Vonlehmden SB, Yang Q, Lee D, Swift LL, Davidson NO, Tso P. Why does the gut choose apolipoprotein B48 but not B100 for chylomicron formation? Am J Physiol Gastrointest Liver Physiol. 2008; 294: G344–G352.