Donate Help Contact The AHA Sign In Home
American Heart Association
Arteriosclerosis, Thrombosis, and Vascular Biology
Search: search_blue_button Advanced Search
Published Online
on December 26, 2008

Arteriosclerosis, Thrombosis, and Vascular Biology. 2008
Published online before print December 26, 2008, doi: 10.1161/ATVBAHA.108.172676
A more recent version of this article appeared on March 1, 2009
This Article
Right arrow Full Text (PDF)
Right arrow Data Supplement
Right arrow All Versions of this Article:
29/3/401    most recent
ATVBAHA.108.172676v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jang, C.
Right arrow Articles by Koh, G. Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jang, C.
Right arrow Articles by Koh, G. Y.

Submitted on June 22, 2008
Accepted on December 1, 2008

Angiopoietin-2 Exocytosis Is Stimulated by Sphingosine-1-Phosphate in Human Blood and Lymphatic Endothelial Cells

Cholsoon Jang ; Young Jun Koh ; Nam Kyu Lim ; Hyun Jung Kang ; Duk Hoon Kim ; Sung Kwang Park ; Gyun Min Lee ; Choon Ju Jeon ; and Gou Young Koh *

From the National Research Laboratory of Vascular Biology and Department of Biological Sciences (C.J., U.J.K., G.M.L., G.Y.K.), KAIST, Daejeon; the Biotherapeutic Division (C.J., N.K.L., H.J.K., C.J.J.), GenExel-Sein Inc, Daejeon; and the Department of Internal Medicine (S.K.P.), Chonbuk National University Medical School, Jeonju, Republic of Korea.

* To whom correspondence should be addressed. E-mail: gykoh{at}kaist.ac.kr.

Objective—Although diverse functions of angiopoietin-2 (Ang2) have been revealed, little is known about upstream signaling molecules regulating Ang2 exocytosis. We therefore investigated the mechanism of Ang2 exocytosis in human blood and lymphatic endothelial cells (BECs and LECs) by stimulation with sphingosine-1-phosphate (S1P).

Methods and Results—By immunostaining and ELISA analyses using our newly developed human Ang2-specific antibodies, Ang2 exocytosis from human endothelial cells was examined. Both exogenous and endogenous S1P trigger rapid Ang2 exocytosis in time- and dose-dependent manners. Intriguingly, S1P-induced Ang2 exocytosis is higher in LECs than BECs. These effects of S1P are mainly mediated by the endothelial differentiation gene receptor 1, which subsequently activates its downstream phospholipase C and intracellular calcium mobilization to trigger Ang2 exocytosis. Consistently, S1P also dramatically stimulates Ang2 exocytosis from the ECs of ex vivo–incubated blood vessels.

Conclusion—These results imply that the rapid secretion of Ang2 by exocytosis from endothelial cells is another possible mechanism underlying S1P-induced angiogenesis and inflammation.
Key words: angiogenesis • angiopoietin • endothelial differentiation gene receptor • lymphangiogenesis • Weibel-Palade bodies




This article has been cited by other articles:


Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
W. P. Dunworth and K. M. Caron
G Protein-Coupled Receptors as Potential Drug Targets for Lymphangiogenesis and Lymphatic Vascular Diseases
Arterioscler Thromb Vasc Biol, May 1, 2009; 29(5): 650 - 656.
[Abstract] [Full Text] [PDF]


ATVB Home | Subscriptions | Archives | Feedback | Authors | Help | AHA Journals Home | Search
Copyright © 2008 American Heart Association, Inc. All rights reserved. Unauthorized use prohibited.