on August 23, 2007
Published online before print August 23, 2007, doi: 10.1161/ATVBAHA.107.151753
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on April 20, 2007
Accepted on August 1, 2007
Cholesterol Accumulation Is Increased in Macrophages of Phospholipid Transfer Protein-Deficient Mice. Normalization by Dietary Alpha-Tocopherol Supplementation
Nicolas Ogier ;
From INSERM U866, Centre de Recherche INSERM, IFR Santé-STIC, Faculté de Médecine, Université de Bourgogne, Dijon, France.
* To whom correspondence should be addressed. E-mail: Catherine.Desrumaux{at}u-bourgogne.fr.
Objective—Phospholipid transfer protein (PLTP) is a multifunctional, extracellular lipid transport protein that plays a major role in lipoprotein metabolism and atherosclerosis. Recent in vivo studies suggested that unlike systemic PLTP, macrophage-derived PLTP would be antiatherogenic. The present study aimed at characterizing the atheroprotective properties of macrophage-derived PLTP.
Methods and Results—Peritoneal macrophages were isolated from PLTP-deficient and wild-type mice and their biochemical characteristics were compared. It is shown that macrophages isolated from PLTP-deficient mice have increased basal cholesterol content and accumulate more cholesterol in the presence of LDL compared with wild-type cells. Cholesterol parameters in macrophages of PLTP-deficient mice were normalized by dietary 
-tocopherol supplementation.
Conclusions—The antiatherogenic properties of macrophage-derived PLTP are related at least in part to its ability to reduce cholesterol accumulation in macrophages through changes in the -tocopherol content and oxidative status of the cells.
Key words: phospholipid transfer protein • vitamin E • tocopherol • macrophage
This article has been cited by other articles:
 |
|
 |
|
  J. F. Oram, G. Wolfbauer, C. Tang, W. S. Davidson, and J. J. Albers An Amphipathic Helical Region of the N-terminal Barrel of Phospholipid Transfer Protein Is Critical for ABCA1-dependent Cholesterol Efflux J. Biol. Chem., April 25, 2008; 283(17): 11541 - 11549. [Abstract] [Full Text] [PDF]
|
|