This Article Full Text Full Text (PDF) Additional Materials All Versions of this Article: 28/5/899    most recent ATVBAHA.108.163634v1 Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Xu, S.-Q. Articles by Goldstein, B. J. Search for Related Content PubMed PubMed Citation Articles by Xu, S.-Q. Articles by Goldstein, B. J. Pubmed/NCBI databases Gene GEO Profiles HomoloGene UniGene Compound via MeSH Substance via MeSH

(Arteriosclerosis, Thrombosis, and Vascular Biology. 2008;28:899.)
© 2008 American Heart Association, Inc.

Cell Biology and Signaling

Adiponectin Protects Against Angiotensin II or Tumor Necrosis Factor –Induced Endothelial Cell Monolayer Hyperpermeability

Role of cAMP/PKA Signaling

Shi-Qiong Xu; Kalyankar Mahadev; Xiangdong Wu; Lauren Fuchsel; Sylvia Donnelly; Rosario G. Scalia; Barry J. Goldstein

From the Division of Endocrinology, Diabetes, and Metabolic Diseases, Department of Medicine (S.-Q.X., K.M., X.W., L.F., S.D., B.J.G.), and the Department of Molecular Physiology and Biophysics (R.G.S.), Jefferson Medical College of Thomas Jefferson University, Philadelphia, Pa.

Correspondence to Barry J. Goldstein, MD, PhD, Director, Division of Endocrinology, Diabetes and Metabolic Diseases, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Room 320 Curtis Building, 1015 Walnut Street, Philadelphia, PA 19107. E-mail Barry.Goldstein{at}jefferson.edu

Objective— Angiotensin II (Ang II) and tumor necrosis factor (TNF)- levels increase endothelial permeability, and we hypothesized that adiponectin suppressed these responses in a cAMP-dependent manner.

Methods and Results— The effect of adiponectin on transendothelial electric resistance (TEER) and diffusion of albumin through human umbilical vein and bovine aortic endothelial cell monolayers induced by Ang II (100 nmol/L) or TNF- (5 ng/mL) was measured. Treatment with the globular domain of adiponectin (3 µg/mL) for 16 hours abrogated the adverse TEER effect of TNF- (–35 versus –12 /cm² at 45 minutes, P<0.05) and Ang II (–25 versus –5 /cm² at 45 minutes, P<0.01) and partially suppressed the increased diffusion of albumin with Ang II (40% versus 10% change, P<0.05) or TNF- (40% versus 20% change, P<0.05). Full-length adiponectin also suppressed Ang II–induced monolayer hyperpermeability. Adiponectin treatment also suppressed Ang II–induced increased actin stress fiber development, intercellular gap formation, and β-tubulin disassembly. Adiponectin increased cAMP levels, and its effects were abrogated by inhibition of adenylyl cyclase or cAMP-dependent protein kinase signaling.

Conclusions— Adiponectin protects the endothelial monolayer from Ang II or TNF--induced hyperpermeability by modulating microtubule and cytoskeleton stability via a cAMP/ PKA signaling cascade.

Full-length and globular adiponectin abrogated the hyperpermeability of an endothelial monolayer elicited by TNF- or Ang II, measured by electric resistance or albumin diffusion. Hyperpermeability correlated with actin stress fiber development, intercellular gap formation, and β-tubulin disassembly, which were all suppressed by adiponectin. Adiponectin increased cellular cAMP, and its effects were blocked by inhibition of cAMP/PKA signaling.

Key Words: adiponectin • angiotensin II • tumor necrosis factor- • permeability • endothelial function • signal transduction • cyclic AMP • protein kinase A