This Article Full Text Full Text (PDF) Data Supplement All Versions of this Article: 28/10/1714    most recent ATVBAHA.108.169912v1 Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yun, S. Articles by Haskard, D. O. Search for Related Content PubMed PubMed Citation Articles by Yun, S. Articles by Haskard, D. O.

(Arteriosclerosis, Thrombosis, and Vascular Biology. 2008;28:1714.)
© 2008 American Heart Association, Inc.

Integrative Physiology/Experimental Medicine

Brief Report: Accelerated Atherosclerosis in Low-Density Lipoprotein Receptor-Deficient Mice Lacking the Membrane-Bound Complement Regulator CD59

Sheng Yun; Viola W.Y. Leung; Marina Botto; Joseph J. Boyle; Dorian O. Haskard

From the Bywaters Centre for Vascular Inflammation, National Heart and Lung Institute (S.Y., V.W.Y.L., J.J.B., D.O.H.), the Division of Investigative Sciences (V.W.Y.L., J.J.B.), and the Molecular Genetics and Rheumatology Section, Division of Medicine (M.B.), Imperial College, London, UK.

Correspondence to Professor Dorian O. Haskard, NHLI Cardiovascular Sciences, Imperial College, Hammersmith Hospital, Du Cane Road, London, UK. E-mail d.haskard{at}imperial.ac.uk

Objective— Whereas studies in humans and animal models have suggested a role for complement activation in atherosclerosis, there has been little analysis of the importance of complement regulators. We tested the hypothesis that the terminal pathway inhibitor CD59 plays an essential role in limiting the proinflammatory effects of complement activation.

Methods and Results— CD59 gene targeted mice (CD59a^–/–) mice were crossed with low-density lipoprotein receptor-deficient (Ldlr^–/–) mice. CD59-deficient Ldlr^–/– mice had significantly more extensive en face Sudan IV staining of thoracoabdominal aorta than Ldlr^–/– single knock-outs, both after a low-fat diet (6.51±0.36% versus 2.63±0.56%, P<0.001) or a high-fat diet (17.05±2.15% versus 7.69±1.17%, P<0.004). Accelerated lesion formation in CD59a^–/–/Ldlr^–/– mice on a high-fat diet was associated with increased lesional vascular smooth muscle cell (VSMC) number and fibrous cap formation.

Conclusion— Our data show that CD59 deficiency accelerates the development of lesions and increases plaque VSMC composition. Assuming that the main function of CD59 is to prevent the development of C5b-9 membrane attack complexes, our observations are consistent with the terminal complement pathway having proatherogenic potential in the Ldlr^–/– mouse model, and highlight the importance of complement regulation.

Key Words: atherosclerosis • inflammation • immune system • complement • mice

This article has been cited by other articles:

				V. W.Y. Leung, S. Yun, M. Botto, J. C. Mason, T. H. Malik, W. Song, D. Paixao-Cavalcante, M. C. Pickering, J. J. Boyle, and D. O. Haskard Decay-Accelerating Factor Suppresses Complement C3 Activation and Retards Atherosclerosis in Low-Density Lipoprotein Receptor-Deficient Mice Am. J. Pathol., October 1, 2009; 175(4): 1757 - 1767. [Abstract] [Full Text] [PDF]

				G. Wu, W. Hu, A. Shahsafaei, W. Song, M. Dobarro, G. K. Sukhova, R. R. Bronson, G.-p. Shi, R. P. Rother, J. A. Halperin, et al. Complement Regulator CD59 Protects Against Atherosclerosis by Restricting the Formation of Complement Membrane Attack Complex Circ. Res., February 27, 2009; 104(4): 550 - 558. [Abstract] [Full Text] [PDF]