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(Arteriosclerosis, Thrombosis, and Vascular Biology. 2007;27:1340.)
© 2007 American Heart Association, Inc.

Atherosclerosis and Lipoproteins

In Vivo Evidence for a Role of Adipose Tissue SR-BI in the Nutritional and Hormonal Regulation of Adiposity and Cholesterol Homeostasis

Laurent Yvan-Charvet; Alexandre Bobard; Pascale Bossard; Florence Massiéra; Xavier Rousset; Gérard Ailhaud; Michèle Teboul; Pascal Ferré; Georges Dagher; Annie Quignard-Boulangé

From INSERM (L.Y-C., A.B., P.B., P.F., G.D., A.Q-B.), U671, Centre Biomédical des Cordeliers, Paris, France; Université Pierre et Marie Curie-Paris6 UMR-S 671, Paris, France; INSERM (X.R.), U505, Centre Biomédical des Cordeliers, Paris, France; CNRS UMR6543 (F.M., G.A., M.T.), Centre National de la Recherche Scientifique, Nice, France.

Correspondence to Laurent Yvan-Charvet., INSERM, U671, 15 rue de l’Ecole de Médecine, 75270 Paris Cedex 06, France. E-mail ly2159{at}columbia.edu

Objectives— This study examines the role of insulin and angiotensin II in high-density lipoprotein (HDL) metabolism by focusing on the regulation and function of scavenger receptor type-BI (SR-BI) in adipose tissue.

Methods and Results— Insulin or angiotensin II injection in wild-type mice induced a decrease in circulating HDL and it was associated with the translocation of SR-BI from intracellular sites to the plasma membrane of adipose tissue. Refeeding upregulated adipose HDL selective cholesteryl esters uptake and SR-BI proteins through transcriptional and posttranscriptional mechanisms. This occurred along with a decrease in serum HDL and an increase in adipose cholesterol content. Similar results were obtained with transgenic mice overexpressing locally angiotensinogen in adipose tissue. In adipose 3T3-L1 cell line, HDL induced lipogenesis by increasing liver X receptor binding activity. This mechanism was dependent of insulin and angiotensin II.

Conclusions— Our results raise the possibility that adipose tissue SR-BI translocation might be a link between adipose tissue lipid storage and HDL clearance.

This study shows that insulin and angiotensin II have a major role in HDL metabolism in vivo. These hormones induce redistribution of SR-BI to the plasma membrane in adipose tissue. This will then lead to an increase in LXR binding activity in an insulin and angiotensin II dependent fashion.

Key Words: adipose tissue • angiotensin II • high-density lipoprotein • insulin

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