This Article Full Text Full Text (PDF) Data Supplement All Versions of this Article: 27/6/1340    most recent ATVBAHA.106.136382v1 Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yvan-Charvet, L. Articles by Quignard-Boulangé, A. Search for Related Content PubMed PubMed Citation Articles by Yvan-Charvet, L. Articles by Quignard-Boulangé, A.

(Arteriosclerosis, Thrombosis, and Vascular Biology. 2007;27:1340.)
© 2007 American Heart Association, Inc.

Atherosclerosis and Lipoproteins

In Vivo Evidence for a Role of Adipose Tissue SR-BI in the Nutritional and Hormonal Regulation of Adiposity and Cholesterol Homeostasis

Laurent Yvan-Charvet; Alexandre Bobard; Pascale Bossard; Florence Massiéra; Xavier Rousset; Gérard Ailhaud; Michèle Teboul; Pascal Ferré; Georges Dagher; Annie Quignard-Boulangé

From INSERM (L.Y-C., A.B., P.B., P.F., G.D., A.Q-B.), U671, Centre Biomédical des Cordeliers, Paris, France; Université Pierre et Marie Curie-Paris6 UMR-S 671, Paris, France; INSERM (X.R.), U505, Centre Biomédical des Cordeliers, Paris, France; CNRS UMR6543 (F.M., G.A., M.T.), Centre National de la Recherche Scientifique, Nice, France.

Correspondence to Laurent Yvan-Charvet., INSERM, U671, 15 rue de l’Ecole de Médecine, 75270 Paris Cedex 06, France. E-mail ly2159{at}columbia.edu

Objectives— This study examines the role of insulin and angiotensin II in high-density lipoprotein (HDL) metabolism by focusing on the regulation and function of scavenger receptor type-BI (SR-BI) in adipose tissue.

Methods and Results— Insulin or angiotensin II injection in wild-type mice induced a decrease in circulating HDL and it was associated with the translocation of SR-BI from intracellular sites to the plasma membrane of adipose tissue. Refeeding upregulated adipose HDL selective cholesteryl esters uptake and SR-BI proteins through transcriptional and posttranscriptional mechanisms. This occurred along with a decrease in serum HDL and an increase in adipose cholesterol content. Similar results were obtained with transgenic mice overexpressing locally angiotensinogen in adipose tissue. In adipose 3T3-L1 cell line, HDL induced lipogenesis by increasing liver X receptor binding activity. This mechanism was dependent of insulin and angiotensin II.

Conclusions— Our results raise the possibility that adipose tissue SR-BI translocation might be a link between adipose tissue lipid storage and HDL clearance.

This study shows that insulin and angiotensin II have a major role in HDL metabolism in vivo. These hormones induce redistribution of SR-BI to the plasma membrane in adipose tissue. This will then lead to an increase in LXR binding activity in an insulin and angiotensin II dependent fashion.

Key Words: adipose tissue • angiotensin II • high-density lipoprotein • insulin

This article has been cited by other articles:

				P. Rao, Z. H. Huang, and T. Mazzone Angiotensin II Regulates Adipocyte Apolipoprotein E Expression J. Clin. Endocrinol. Metab., November 1, 2007; 92(11): 4366 - 4372. [Abstract] [Full Text] [PDF]