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(Arteriosclerosis, Thrombosis, and Vascular Biology. 2007;27:594.)
© 2007 American Heart Association, Inc.

Atherosclerosis and Lipoproteins

Total Body ABCG1 Expression Protects Against Early Atherosclerotic Lesion Development in Mice

Ruud Out; Menno Hoekstra; Illiana Meurs; Paula de Vos; Johan Kuiper; Miranda Van Eck; Theo J.C. Van Berkel

From the Division of Biopharmaceutics, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, The Netherlands.

Correspondence to R. Out, Division of Biopharmaceutics, Gorlaeus Laboratories, Einsteinweg 55, 2333CC Leiden, The Netherlands. E-mail r.out{at}lacdr.leidenuniv.nl

Objective— ABCG1 has recently been identified as a facilitator of cholesterol and phospholipid efflux from macrophages to HDL. In bone marrow transplantation studies, we and others have now shown that the absence of macrophage ABCG1 may differentially influence atherosclerotic lesions dependent on the experimental setting and/or the stage of atherosclerotic lesion development. To further define the role of ABCG1 in atherogenesis, we investigated in the current study the effect of total body deficiency of ABCG1 on atherosclerotic lesion development.

Methods and Results— ABCG1^–/– mice and wild-type littermates were fed an atherogenic diet for 12 weeks to induce atherosclerotic lesion formation. Both before and after the start of the atherogenic diet, serum lipid levels and lipoprotein profiles did not differ significantly between the two groups. In addition no significant difference in serum apoE levels was found after diet feeding. In wild-type mice the atherogenic diet induced the formation of macrophage-rich early lesions (size: 24±7x10³ µm² [n=6]). Feeding ABCG1^–/– mice the atherogenic diet led to a significant 1.9-fold stimulation of atherosclerotic lesion size (46±6x10³ µm² [n=7]; Student t test P=0.034 and Mann–Whitney test P=0.050) compared with controls, suggesting a clear antiatherogenic role for ABCG1. At the same time, excessive lipid accumulation was observed in macrophage-rich areas of the lungs and spleens of ABCG1^–/– mice as compared with wild-type mice.

Conclusions— Total body ABCG1 expression protects against early atherosclerotic lesion development.

It has been shown that absence of macrophage ABCG1 may differentially influence atherosclerotic lesions dependent on the experimental setting and/or stage of atherosclerotic lesion development. In the current study we show that total body ABCG1 expression protects against early atherosclerotic lesion development.

Key Words: ABCG1 deficiency • atherosclerosis • atherogenic diet • lung • spleen • mice

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