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(Arteriosclerosis, Thrombosis, and Vascular Biology. 2007;27:161.)
© 2007 American Heart Association, Inc.

Atherosclerosis and Lipoproteins

Establishment of a High Sensitivity Plasma Assay for Human Pentraxin3 as a Marker for Unstable Angina Pectoris

Kenji Inoue; Akira Sugiyama; Patrick C. Reid; Yukio Ito; Katsumi Miyauchi; Sei Mukai; Mina Sagara; Kyoko Miyamoto; Hirokazu Satoh; Isao Kohno; Takeshi Kurata; Hiroshi Ota; Alberto Mantovani; Takao Hamakubo; Hiroyuki Daida; Tatsuhiko Kodama

From the Laboratory for Systems Biology and Medicine (K.I., P.C.R., T.H., T.K.), Research Center for Advanced Science and Technology, The University of Tokyo, Japan; the Department of Cardiology (K.I.), Juntendo University Nerima Hospital, Tokyo, Japan; the Perseus Proteomics Inc (A.S., P.C.R., Y.I., M.S., K.M., H.S., I.K.), Tokyo, Japan; the Department of Cardiology (K.M., T.K., H.O., H.D.), Juntendo University School of Medicine, Tokyo, Japan; the Shin Yokohama Cardiovascular Clinic (S.M.), Kanagawa, Japan; and the Istituto Clinico Humanitas (A.M.), Italy.

Correspondence to Tatsuhiko Kodama, Laboratory for Systems Biology and Medicine, Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1, Komaba, Meguro, Tokyo, 153-8904, Japan. E-mail kodama{at}lsbm.org

Objective— Plasma pentraxin 3 (PTX3) levels are increased in patients with acute myocardial infarction, yet its involvement in unstable angina pectoris (UAP) remains unclear. To critically evaluate the role of PTX3 in UAP, a sensitive and precise measurement of PTX3 concentration is needed.

Methods and Results— We established a high sensitive plasma ELISA assay system for the detection of PTX3 using monoclonal antibodies. The lower limit of detection of our ELISA was 0.1 ng/mL, sensitivity far greater than the current commercially available kit. Plasma samples were obtained from 162 consecutive patients treated for hypertension, hyperlipidemia, diabetes mellitus, or cardiovascular disease at a physician’s office. PTX3 was not associated with any known coronary risk factors. Additionally, we collected plasma samples from 252 consecutive subjects admitted to a university hospital for coronary artery assessment by coronary angiography. PTX3 was significantly increased in patients in whom coronary intervention was performed. We further analyzed the plasma level of PTX3 in 52 patients with effort angina (EAP) and 16 patients with UAP. Compared with the control group, PTX3 were significantly higher in the UAP group.

Conclusions— The levels of plasma PTX3 were increased in patients with arterial inflammation, especially UAP. This PTX3 detection system will be useful for the prediction of UAP.

Pentraxin 3 (PTX3) are increased in patients with acute myocardial infarction, yet its involvement in unstable angina (UAP) remains unclear. We developed a high sensitivity ELISA system for the detection of PTX3. PTX3 were increased in patients with UAP. This ELISA system will be useful for the prediction of UAP.

Key Words: PTX3 • hs-CRP • atherosclerosis • inflammation • acute coronary syndrome

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