Published online before print March 30, 2006, doi: 10.1161/01.ATV.0000219687.71607.f7
© 2006 American Heart Association, Inc.
Atherosclerosis and Lipoproteins |
A Simple Method of Plaque Rupture Induction in Apolipoprotein E–Deficient Mice
From the Department of Geriatrics (T.S., M.K., K.N., X.W.C., T.S., A.I.), Nagoya University Graduate School of Medicine, Japan; and Department of Anatomy and Neuroscience (T.S., K.S.), Hamamatsu University School of Medicine, Shizuoka, Japan.
Correspondence to Masafumi Kuzuya, Department of Geriatrics, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya, Japan. E-mail kuzuya{at}med.nagoya-u.ac.jp
Objective— The development of a murine model of atherosclerotic plaque rupture.
Methods and Results— The left common carotid arteries of male apolipoprotein E (apoE)–deficient mice (9 weeks old) were ligated just proximal to their bifurcations. After 4 weeks on a standard diet, the mice received polyethylene cuff placement just proximal to the ligated site, and the animals were then processed for morphological studies at specific time points. Ligation of the carotid artery in apoE-deficient mice for 4 weeks induced marked intimal hyperplasia, which is a lipid- and collagen-rich lesion that contains a number of macrophages, T lymphocytes, and smooth muscle cells. Subsequently, the cuff placement evoked intraplaque hemorrhage and plaque rupture with fibrin(ogen)-positive luminal thrombus in this region accompanying a decrease in collagen content as well as an increase in apoptotic cells in the intima within a few days after cuff placement.
Conclusions— We demonstrated the murine model of human plaque rupture, which is simple, fast, and highly efficient. This model would help us not only to understand the mechanism of human plaque rupture but also to assess various already-known and as-yet-unknown agents in the future.
In this study we demonstrate the murine model of human plaque rupture, which is simple, fast, and highly efficient. This model would help us not only to understand the mechanism of human plaque rupture but also to assess various already-known and as-yet-unknown agents in the future.
Key Words: animal model • plaque rupture • thrombus • apoptosis • collagen
Related Article:
Arterioscler Thromb Vasc Biol 2006 26: 1191-1192.
This article has been cited by other articles:
 |
|
 |
|
  M Ni, W Q Chen, and Y Zhang Animal models and potential mechanisms of plaque destabilisation and disruption Heart, September 1, 2009; 95(17): 1393 - 1398. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z.-S. Shi, L. Feng, X. He, A. Ishii, J. Goldstine, H.V. Vinters, and F. Vinuela Vulnerable Plaque in a Swine Model of Carotid Atherosclerosis AJNR Am. J. Neuroradiol., March 1, 2009; 30(3): 469 - 472. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Schonberger, D. Siegel-Axel, R. Bussl, S. Richter, M. S. Judenhofer, R. Haubner, G. Reischl, K. Klingel, G. Munch, P. Seizer, et al. The immunoadhesin glycoprotein VI-Fc regulates arterial remodelling after mechanical injury in ApoE-/- mice Cardiovasc Res, October 1, 2008; 80(1): 131 - 137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-S. Kuo, J. M. Kalbfleisch, P. Rutherford, D. Gifford-Moore, X.-d. Huang, R. Christie, K. Hui, K. Gould, and M. Rekhter Chemical analysis of atherosclerotic plaque cholesterol combined with histology of the same tissue J. Lipid Res., June 1, 2008; 49(6): 1353 - 1363. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. van Leeuwen, M. J.J. Gijbels, A. Duijvestijn, M. Smook, M. J. van de Gaar, P. Heeringa, M. P.J. de Winther, and J. W. C. Tervaert Accumulation of Myeloperoxidase-Positive Neutrophils in Atherosclerotic Lesions in LDLR / Mice Arterioscler Thromb Vasc Biol, January 1, 2008; 28(1): 84 - 89. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Westrick, M. E. Winn, and D. T. Eitzman Murine Models of Vascular Thrombosis Arterioscler Thromb Vasc Biol, October 1, 2007; 27(10): 2079 - 2093. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Doyle and N. Caplice Plaque Neovascularization and Antiangiogenic Therapy for Atherosclerosis J. Am. Coll. Cardiol., May 29, 2007; 49(21): 2073 - 2080. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. V. Denis and D. D. Wagner Platelet Adhesion Receptors and Their Ligands in Mouse Models of Thrombosis Arterioscler Thromb Vasc Biol, April 1, 2007; 27(4): 728 - 739. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Jackson, M. R. Bennett, E. A.L. Biessen, J. L. Johnson, and R. Krams Assessment of Unstable Atherosclerosis in Mice Arterioscler Thromb Vasc Biol, April 1, 2007; 27(4): 714 - 720. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Falk, S. M. Schwartz, Z. S. Galis, and M. E. Rosenfeld Neointimal Cracks (Plaque Rupture?) and Thrombosis in Wrapped Arteries Without Flow Arterioscler Thromb Vasc Biol, January 1, 2007; 27(1): 248 - 249. [Full Text] [PDF]
|
|
|
|
|
|
C. L. Jackson Neointimal Cracks (Plaque Rupture?) and Thrombosis in Wrapped Arteries Without Flow Arterioscler Thromb Vasc Biol, January 1, 2007; 27(1): 250 - 251. [Full Text] [PDF]
|
|
|
|
|
|
M. Kuzuya and T. Sasaki Neointimal Cracks (Plaque Rupture?) and Thrombosis in Wrapped Arteries Without Flow Arterioscler Thromb Vasc Biol, January 1, 2007; 27(1): 252 - 252. [Full Text] [PDF]
|
|
|
|
|
|
C. L. Jackson Ruptures of Delight?: A New Mouse Model of Plaque Rupture. Arterioscler Thromb Vasc Biol, June 1, 2006; 26(6): 1191 - 1192. [Full Text] [PDF]
|
|