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(Arteriosclerosis, Thrombosis, and Vascular Biology. 2006;26:1043.)
© 2006 American Heart Association, Inc.

Vascular Biology

Homocysteine Activates cAMP-response Element Binding Protein in HepG2 Through cAMP/PKA Signaling Pathway

Connie W.H. Woo; Yaw L. Siow; Karmin O

From the Department of Animal Science, Department of Physiology, University of Manitoba, Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Research Centre, Winnipeg, Canada.

Correspondence to Dr Karmin O, MD, PhD, Laboratory of Integrative Biology, St. Boniface Hospital Research Centre, R4032, 351 Tache Avenue, Winnipeg, Manitoba, Canada R2H 2A6. E-mail karmino{at}sbrc.ca

Objective— Hyperhomocysteinemia is an independent risk factor for cardiovascular disorders. Our previous studies demonstrated that hyperhomocysteinemia not only elicited inflammatory responses in the vascular endothelium but also induced fatty liver and hypercholesterolemia via transcriptional regulation. One of the transcription factors activated in the liver during hyperhomocysteinemia was cAMP-response element binding protein (CREB). CREB regulates the expression of many genes including those involved in lipid and glucose metabolism. In this study, we investigated the molecular mechanism by which Hcy activated CREB in rat liver and in hepatocytes (HepG2).

Method and Results— Hyperhomocysteinemia was induced in rats by feeding high-methionine diet for 4 weeks. There was a significant increase in hepatic cAMP levels, protein kinase A (PKA) activity and an activation of CREB. Incubation of HepG2 cells with Hcy (50 to 100 µmol/L) significantly enhanced CREB phosphorylation and subsequently increased CREB/DNA binding activity. PKA was activated in Hcy-treated cells as a result of increased cellular cAMP level. Inhibition of adenylyl cyclase not only reduced the intracellular cAMP levels elevated by Hcy treatment but also inhibited PKA activation and prevented Hcy-induced CREB phosphorylation.

Conclusion— These results suggest that the cAMP/PKA signaling pathway plays an important role in mediating Hcy-induced CREB activation in hepatocyte.

We investigated the effect of hyperhomocysteinemia on hepatic CREB activation and the underlying mechanism. Homocysteine stimulated adenylyl cyclase activity leading to increased cAMP production and subsequently PKA activation in rat liver and hepatocytes (HepG2). PKA activation was responsible for Hcy-induced phosphorylation of CREB followed by activation of this transcription factor

Key Words: cAMP • CREB • homocysteine • HMG-CoA reductase • PKA

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