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(Arteriosclerosis, Thrombosis, and Vascular Biology. 2005;25:1659.)
© 2005 American Heart Association, Inc.

Atherosclerosis and Lipoproteins

Reduced Immunoregulatory CD31⁺ T Cells in the Blood of Atherosclerotic Mice With Plaque Thrombosis

Giuseppina Caligiuri; Emilie Groyer; Jamila Khallou-Laschet; Ayman Al Haj Zen; Julie Sainz; Dominique Urbain; Anh-Thu Gaston; Mathilde Lemitre; Antonino Nicoletti; Antoine Lafont

From EMI0016 (G.C., A.A.H.Z., J.S., D.U., M.L., A.L.), Faculté de Médecine Paris, France; U681 (E.G., J.K.-L., A.-T.G., A.N.), Institut des Cordeliers, INSERM, Paris, France.

Correspondence to Giuseppina Caligiuri, MD, PhD, INSERM U681, Institut de Cordeliers; 15, rue de l’Ecole de Médecine, 75006 Paris, France. E-mail caligiuri{at}necker.fr or giuseppina.Caligiuri@u430.bhdc.jussieu.fr

Objective— Lymphocyte activation is thought to play a major role in the pathogenesis of atherosclerotic complications such as plaque thrombosis. Circulating CD31⁺ T cells have been shown to regulate human T cell activation. Aim of this study was to evaluate whether the proportion of circulating immunoregulatory CD31⁺ T cells is correlated to the occurrence of plaque thrombosis in aged apolipoprotein (apo) E knockout (KO) mice.

Methods and Results— CD31⁺ T cell depletion of spleen T cells enhanced proliferation (P<0.05) and interferon- production (P<0.01) while reducing interleukin (IL)-4 (P<0.001) and IL-10 (P=0.001) secretion in response to minimally modified low-density lipoprotein. CD31⁺ T cells were counted in 65 apoE KO mice (46-week-old) by flow cytometry. Organizing thrombi could be documented in 28 of 195 (14%) lesions and in at least one of the aorta root lesions in 23 of 65 mice (35%). CD31⁺ T cell count was significantly reduced in mice showing plaque thrombosis (72.3±1.5% versus 84.1±1.2%; P<0.0001), but such reduction did not follow induced plaque rupture or experimentally controlled thrombosis.

Conclusions— Reduced CD31⁺ T cells in circulating blood is a hallmark of atherosclerotic plaque thrombosis. Our data suggest that CD31⁺ T cells may play an important regulatory role in the development of plaque thrombosis.

Lymphocyte activation plays a major role in the pathogenesis of plaque thrombosis. CD31⁺ T cells regulate T cells activation. We found that circulating CD31⁺ T cells were reduced in apolipoprotein E knockout mice with plaque thrombosis. Our data suggest that CD31⁺ T cells may play a regulatory role in plaque thrombosis.

Key Words: atherosclerosis • lymphocytes • thrombosis

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