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(Arteriosclerosis, Thrombosis, and Vascular Biology. 2004;24:1659.)
© 2004 American Heart Association, Inc.

Vascular Biology

Correlation of NO Metabolites and 8-Iso-Prostaglandin F_2a With Periventricular Hyperintensity Severity

Hiroshi Shibata; Toru Nabika; Hidehiko Moriyama; Junichi Masuda; Shotai Kobayashi

From the Departments of Laboratory Medicine (H.S., J.M.) and Functional Pathology (T.N.), Central Clinical Laboratory (H.M.), and Third Department of Internal Medicine (S.K.), School of Medicine, Shimane University, Izumo, and Shimane Institute of Health Science (T.N., J.M., S.K.), Izumo, Japan.

Correspondence to Toru Nabika, MD, Department of Functional Pathology, School of Medicine, Shimane University, Izumo 693-8501, Japan. E-mail nabika{at}med.shimane-u.ac.jp

Objective— Oxidative stress and NO are thought to play important roles in arteriosclerosis pathogenesis, a major cause of white matter lesions in the brain. Therefore, we examined whether NO metabolites (NOx) and 8-iso-prostaglandin F₂ (IsoP) levels in vivo correlated with the severity of periventricular hyperintensity (PVH) to evaluate potential roles of oxidative stress and NO in white matter lesions.

Methods and Results— Participants (687 males and 528 females) of a health-screening examination were recruited into the study. The plasma NOx and urinary IsoP levels were measured using the Griess method and ELISA, respectively. PVH was diagnosed on the basis of MRIs. In nonparametric univariate trend analyses, plasma NOx as well as aging, presence of hypertension and of lacunes, mean blood pressure, and high-density lipoprotein cholesterol showed highly significant monotone correlation with PVH severity (P0.01). By the multivariate ordinal regression analysis, the plasma NOx (P=0.002) and urinary IsoP (P=0.01) levels were found to be independent factors influencing the severity of PVH together with aging (P<0.001), presence of hypertension (P<0.001) and lacunes (P<0.001), and mean blood pressure (P=0.001).

Conclusions— Oxidative stress and NO have a close correlation with PVH severity.

Oxidative stress and NO levels were evaluated in a general population with or without mild periventricular hyperintensity under a cross-sectional study design. Serum NOx (NO metabolites) and urinary 8-iso-PG F₂ (a marker for oxidative stress) correlated with the severity of periventricular hyperintensity in a multivariate analysis.

Key Words: lacunar infarction • nitric oxide • oxidative stress • white matter • small-vessel disease