Donate Help Contact The AHA Sign In Home
American Heart Association
Arteriosclerosis, Thrombosis, and Vascular Biology
Search: search_blue_button Advanced Search
« Previous Article | Table of Contents | Next Article »
Arteriosclerosis, Thrombosis, and Vascular Biology. 2004;24:975-981
Published online before print March 25, 2004, doi: 10.1161/01.ATV.0000126681.16619.69
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
24/5/975    most recent
01.ATV.0000126681.16619.69v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Paffen, E.
Right arrow Articles by Bertina, R. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Paffen, E.
Right arrow Articles by Bertina, R. M.
Related Collections
Right arrow Cell biology/structural biology
Right arrow Arterial thrombosis
Right arrow Coagulation
Right arrow Acute myocardial infarction
Right arrow Mechanism of atherosclerosis/growth factors
(Arteriosclerosis, Thrombosis, and Vascular Biology. 2004;24:975.)
© 2004 American Heart Association, Inc.

Thrombosis

C-Reactive Protein Does Not Directly Induce Tissue Factor in Human Monocytes

Elaine Paffen; Hans L. Vos; Rogier M. Bertina

From the Hemostasis and Thrombosis Research Centre, Department of Hematology, Leiden University Medical Centre, Leiden, the Netherlands.

Correspondence to Elaine Paffen, Hemostasis and Thrombosis Research Centre, Department of Hematology, Leiden University Medical Centre, Albinusdreef 2, 2333 ZA Leiden, the Netherlands. E-mail epaffen{at}lumc.nl

Objective— It is generally assumed that C-reactive protein (CRP) induces synthesis of tissue factor (TF) in monocytic cells, thereby potentially initiating intravascular blood coagulation. We aimed to elucidate the mechanism of CRP-induced TF expression in monocytes and monocyte-derived macrophages (MDMs) in vitro.

Methods and Results— Monocytes were isolated from the blood of healthy donors and cultured with or without CRP or lipopolysaccharide (LPS) to study the time course of TF antigen and TF mRNA expression. Addition of 100 µg/mL CRP did not result in a significant increase in TF antigen (range: 9 to 163 pg/106 cells, n=11) and TF mRNA (relative number of TF transcripts; NTF=0.01 to 0.33), when compared with nonstimulated cells (TF antigen 7 to 46 pg/106 cells, NTF=0.01 to 0.13). Variation of CRP concentration and exposure time did not affect the TF response. Similar results were obtained in monocytes cultured in suspension and in MDMs. In contrast, TF was strongly induced by 10 µg/mL LPS (TF antigen 1125 to 6120 pg/106 cells, NTF=5.94 to 23.43). Cultured monocytes did express FcR{gamma}II, a putative CRP receptor, and addition of CRP induced a 7-fold increase in the production of monocyte chemoattractant protein-1 (MCP-1). Interestingly, CRP addition to peripheral blood mononuclear cells (PBMCs) did result in TF expression on monocytic cells.

Conclusions— The absence of TF induction after incubation of purified monocytes with CRP indicates that CRP is unable to induce TF expression in monocytes and MDMs directly. The presence of CRP-induced TF expression in PBMCs suggests that CRP can induce TF indirectly, probably through cross-talk between cells.


Key Words: C-reactive protein • tissue factor • monocytes • MCP-1 • atherosclerosis




This article has been cited by other articles:


Home page
 Clin. Chem.Home page
S. Devaraj, U. Singh, and I. Jialal
The Evolving Role of C-Reactive Protein in Atherothrombosis
Clin. Chem., February 1, 2009; 55(2): 229 - 238.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
M. A. Sardo, S. Campo, G. Mandraffino, C. Saitta, A. Bonaiuto, M. Castaldo, M. Cinquegrani, G. Pizzimenti, and A. Saitta
Tissue Factor and Monocyte Chemoattractant Protein-1 Expression in Hypertensive Individuals with Normal or Increased Carotid Intima-Media Wall Thickness
Clin. Chem., May 1, 2008; 54(5): 814 - 823.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Cai, C. Song, I. Endoh, J. Goyette, W. Jessup, S. B. Freedman, H. P. McNeil, and C. L. Geczy
Serum Amyloid A Induces Monocyte Tissue Factor
J. Immunol., February 1, 2007; 178(3): 1852 - 1860.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
E. Paffen and M. P.M. deMaat
C-reactive protein in atherosclerosis: A causal factor?
Cardiovasc Res, July 1, 2006; 71(1): 30 - 39.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
B. M. Scirica, D. A. Morrow, S. Verma, S. Devaraj, I. Jialal, B. M. Scirica, D. A. Morrow, S. Verma, S. Devaraj, and I. Jialal
The Verdict Is Still Out
Circulation, May 2, 2006; 113(17): 2128 - 2151.
[Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
Y. Zhang and L. M. Wahl
Synergistic enhancement of cytokine-induced human monocyte matrix metalloproteinase-1 by C-reactive protein and oxidized LDL through differential regulation of monocyte chemotactic protein-1 and prostaglandin E2
J. Leukoc. Biol., January 1, 2006; 79(1): 105 - 113.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
A. Pandolfi
C-reactive protein: A potential new molecular link between inflammation, thrombosis and vascular cell proliferation?
Cardiovasc Res, October 1, 2005; 68(1): 3 - 4.
[Full Text] [PDF]


ATVB Home | Subscriptions | Archives | Feedback | Authors | Help | AHA Journals Home | Search
Copyright © 2004 American Heart Association, Inc. All rights reserved. Unauthorized use prohibited.