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(Arteriosclerosis, Thrombosis, and Vascular Biology. 2004;24:376.)
© 2004 American Heart Association, Inc.

Thrombosis

Platelets of Female Mice Are Intrinsically More Sensitive to Agonists Than Are Platelets of Males

Xing-Hong Leng; Song Yun Hong; Susana Larrucea; Wei Zhang; Tong-Tong Li; José A. López; Paul F. Bray

From Medicine/Thrombosis Research, Baylor College of Medicine, Houston, TX.

Correspondence to Dr Paul F. Bray, Thrombosis Research Section, Baylor College of Medicine, One Baylor Plaza, BCM 286, N1319, Houston, TX 77030. E-mail pbray{at}bcm.tmc.edu

Objective— It has been reported that women fare worse after ischemic coronary events, but the mechanisms remain unclear. Because platelets play a central role in the formation of occlusive thrombi at sites of ruptured atherosclerotic plaques, we studied male/female paired mouse littermates for sex differences in platelet function.

Methods and Results— We compared platelet reactivity in male/female mouse littermates by monitoring agonist-induced fibrinogen (FGN) binding and platelet aggregation. Compared with the platelets from males, platelets from females bound more FGN in response to low concentrations of thrombin and collagen-related peptide. Female platelets also demonstrated greater aggregation in response to adenosine diphosphate and collagen-related peptide. Platelet protein tyrosine phosphorylation on activation also showed small differences between sexes. These differences are independent of platelet size and surface expression of _IIbß₃ and GPIb-IX-V, and they were not blocked by apyrase or aspirin. The sex differences we observed were intrinsic to platelets, because they were observed in washed platelets, but not when platelets were in plasma.

Conclusions— The platelets of female mice were more reactive than those of males in a manner independent of COX-1 and secreted ADP.

Key Words: platelet • thrombin • collagen-related peptide

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