Arteriosclerosis, Thrombosis, and Vascular Biology. 2004;24:288-293
Published online before print December 29, 2003, doi: 10.1161/01.ATV.0000114236.77009.06
Published online before print December 29, 2003, doi: 10.1161/01.ATV.0000114236.77009.06
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© 2004 American Heart Association, Inc.
Vascular Biology |
Characterization of Two Types of Endothelial Progenitor Cells and Their Different Contributions to Neovasculogenesis
From the Cardiovascular Laboratory (J.H., C.-H.Y., H.-S.K., J.-H.C., H.-J.K., K.-K.H.), Clinical Research Institute, Seoul National University Hospital, Korea; and the Department of Internal Medicine (J.H., C.-H.Y., H.-S.K., J.-H.C., H.-J.K., K.-K.H., B.-H.O., M.-M.L., Y.-B.P.), Seoul National University College of Medicine, Seoul, Korea.
Correspondence to Dr Hyo-Soo Kim or Dr Young-Bae Park, Department of Internal Medicine, Seoul National University College of Medicine, 28 Yongon-dong Chongno-gu, Seoul 110-744, Korea. E-mail hyosoo{at}snu.ac.kr
Objective— Endothelial progenitor cells (EPC) in one study group is not the same as EPC in other investigators, suggesting that EPC is not a single type of cell population. In this study, we tried to demonstrate the heterogeneity of EPC.
Methods and Results— We cultured total mononuclear cells from human peripheral blood to get two types of EPC sequentially from the same donors. We called them early EPC and late EPC. Early EPC with spindle shape showed peak growth at 2 to 3 weeks and died at 4 weeks, whereas late EPC with cobblestone shape appeared late at 2 to 3 weeks, showed exponential growth at 4 to 8 weeks, and lived up to 12 weeks. Late EPC was different from early EPC in the expression of VE-cadherin, Flt-1, KDR, and CD45. Late EPC produced more nitric oxide, incorporated more readily into human umbilical vein endothelial cells monolayer, and formed capillary tube better than early EPC. Early EPC secreted angiogenic cytokines (vascular endothelial growth factor, interleukin 8) more so than late EPC during culture in vitro. Both types of EPC showed comparable in vivo vasculogenic capacity.
Conclusions— We found two types of EPC from a source of adult peripheral blood that might have different roles in neovasculogenesis based on the identified differences.
Key Words: stem cells • endothelial progenitor cell • endothelial cell • angiogenesis • cell therapy
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