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(Arteriosclerosis, Thrombosis, and Vascular Biology. 2003;23:1960.)
© 2003 American Heart Association, Inc.

Editorials

HPRT Targeting

"Ets" A Powerful Tool For Investigating Endothelial-Cell Specific Gene Expression

Michael J. Ryan; Curt D. Sigmund

From the Department of Medicine and Physiology and Biophysics, Roy J. and Lucille A. Carver College of Medicine, The University of Iowa, Iowa City.

Address correspondence to Curt D. Sigmund, PhD, Professor Department of Internal Medicine, 3181 MERF, University of Iowa, Department of Internal Medicine, Iowa City, IA 52242. E-mail Curt-Sigmund@uiowa.edu

An extract of the first 250 words of the full text is provided, because this article has no abstract.

Elucidating the role of genes that are important for the development of the vascular endothelium and their time course of expression throughout ontogeny is an important and exciting area of ongoing investigation. In addition to advances that can be made to better understand the vasculature from a developmental perspective, there are obvious clinical implications from determining how gene expression in the endothelium participates in blood vessel formation and function. For example, since tumors require an adequate supply of blood to proliferate, there is a tremendous interest in uncovering mechanisms controlling vascularization and angiogenesis. Moreover, enhancing our understanding of the regulation of gene expression in the blood vessel wall has clear clinical implications for improving collateral blood vessel formation in tissues after an ischemic insult and in peripheral vascular disease.

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One of the most common methods to experimentally examine how a gene is regulated is by "promoter bashing." Promoter bashing is usually performed by transient transfection of recombinant DNA constructs consisting of the 5' flanking region of the gene of interest fused to easily assayable reporter genes such as luciferase or LacZ. The presumption is that the 5' flanking sequence contains regulatory elements vital to the control of transcriptional activity that are required for targeting cell-specific expression. Systematic deletions or mutations created in the construct with subsequent evaluation of their effects on reporter gene activity, coupled with molecular assays designed to identify cognate transcription factors, has been a powerful approach to identify regulatory elements and proteins regulating gene . . . [Full Text of this Article]

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