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(Arteriosclerosis, Thrombosis, and Vascular Biology. 2003;23:1934.)
© 2003 American Heart Association, Inc.

Thrombosis

Platelet Adhesion to Collagen and Collagen-Related Peptide Under Flow

Roles of the ₂ß₁ Integrin, GPVI, and Src Tyrosine Kinases

Renata Polanowska-Grabowska; Jonathan M. Gibbins; Adrian R.L. Gear

From the Department of Biochemistry and Molecular Genetics (R.P.-G., A.R.L.G.), University of Virginia Medical School, Charlottesville, Va, and School of Animal and Microbial Sciences (J.M.G.), University of Reading, UK.

Correspondence to Renata Polanowska-Grabowska, Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908. E-mail rp4t{at}virginia.edu

Objective— Platelet stimulation by collagen and collagen-related peptides (CRPs) is associated with activation of protein tyrosine kinases. In the present study, we investigated the role of Src family tyrosine kinases in the initial adhesion events of human platelets to collagen and cross-linked CRP.

Methods and Results— Under arterial flow conditions, a glycoprotein VI–specific substrate, cross-linked CRP, caused rapid (<2 second) platelet retention and protein tyrosine phosphorylation that were markedly decreased by the Src family kinase inhibitor pyrozolopyrimidine (PP2) or by aggregation inhibitor GRGDSP. CRP-induced platelet retention was transient, and 90% of single platelets or aggregates detached within seconds. PP2, although having no effect on RGD peptide–binding to CRP, completely blocked aggregation and tyrosine phosphorylation of Syk and phospholipase C2 (PLC2). In contrast, PP2 weakly (<30%) suppressed firm adhesion to collagen mediated primarily by the ₂ß₁ integrin. Although PP2 prevented activation of Syk and PLC2 in collagen-adherent platelets, tyrosine phosphorylation of several unidentified protein bands persisted, as did autophosphorylation of pp125^FAK.

Conclusions— These findings indicate that activation of Src-tyrosine kinases Syk and PLC2 is not required for the initial stable attachment of human platelets to collagen and for FAK autophosphorylation. However, Src-tyrosine kinases are critical for glycoprotein VI–mediated signaling leading to platelet aggregation.

Key Words: platelets • adhesion • collagen • Collagen-related peptide • protein tyrosine kinase • flow