Published online before print June 19, 2003, doi: 10.1161/01.ATV.0000082525.84814.A9
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© 2003 American Heart Association, Inc.
Atherosclerosis and Lipoproteins |
Association Between a Novel 11–Base Pair Deletion Mutation in the Promoter Region of the Scavenger Receptor Class B Type I Gene and Plasma HDL Cholesterol Levels in Taiwanese Chinese
From the First Cardiovascular Division, Department of Internal Medicine, Chang Gung Memorial Hospital, Taipei, Taiwan.
Correspondence and reprint requests to Yu-Lin Ko, MD, PhD, First Cardiovascular Division, Department of Internal Medicine, Chang Gung Memorial Hospital, No. 199, Tung-Hwa North Rd, Taipei, Taiwan. E-mail koyulin{at}adm.cgmh.org.tw
Objective— Scavenger receptor class B type I (SR-BI) is a multiligand cell-surface receptor that mediates the selective uptake of lipid from HDL cholesterol (HDL-C) into cells. This study hypothesized an association between functional variants in the promoter region of SR-BI gene and HDL-C levels.
Methods and Results— We identified 2 novel mutations in the SR-BI gene promoter region by using single-strand conformation polymorphism. One mutation was an 11-bp CCCCGCCCCGT deletion mutation from positions -140 to -150 relative to the transcription start site, corresponding to an Sp1 binding site; the other was a C
T substitution at position -142. Twenty-six of 690 unrelated subjects were heterozygous for the -140 to -150 deletion mutation, and the allele frequency in this population was 0.02. This study showed that the deletion variant prevented binding of Sp1 to this region of the SR-BI promoter and effectively reduced transcriptional activities in HepG2 cells. Notably, the -140 to -150 deletion mutation was significantly associated with increased HDL-C levels and explained 
0.5% of the variation in HDL-C levels in this population.
Conclusions— A genetic variant at the SR-BI gene promoter region might explain a significant proportion of individual differences in HDL-C levels among Taiwanese Chinese. Our results require further replication in an independent population.
Key Words: deletion • mutation • scavenger receptor class B type I • HDL cholesterol
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