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(Arteriosclerosis, Thrombosis, and Vascular Biology. 2003;23:148.)
© 2003 American Heart Association, Inc.

Letters to the Editor

Expression of CXCL16 in Human T Cells

P. Shashkin; D. Simpson; V. Mishin; B. Chesnutt; K. Ley

Cardiovascular Research Center, University of Virginia, Charlottesville, Va

An extract of the first 250 words of the full text is provided, because this article has no abstract.

To the Editor:

In the November 2001 issue of Arteriosclerosis, Thrombosis, and Vascular Biology, Minami et al¹ demonstrated expression of a novel scavenger receptor for phosphatidylserine and oxidized lipoprotein (SR-PSOX), which is identical to the chemokine CXCL16, in lipid-laden macrophages accumulated in the intima of atherosclerotic lesions. Based on reports of this group as well as reports of other authors,^2–4 SR-PSOX expression has been shown to be a unique feature of antigen-presenting cells (macrophages, dendritic cells, and CD19-positive B lymphocytes) but not of other cells. However, in the April 2002 issue of Arteriosclerosis, Thrombosis, and Vascular Biology, Hofnagel et al⁵ used reverse-transcription–polymerase chain reaction (RT-PCR) to detect expression of SR-PSOX in smooth muscle cells and endothelial cells, thus raising the question whether SR-PSOX/CXCL16 may be expressed in other cells besides antigen-presenting cells. These authors also observed a lack of regulation of this ligand by tumor necrosis factor- and by other proinflammatory stimuli, calling into question the mechanisms activating CXCL16 expression.⁵

Using quantitative real-time RT-PCR, we found expression of CXCL16-specific mRNA in human T cells. Human peripheral blood was collected from antecubital vein of healthy donors. White blood cells were isolated by centrifugation on Histopaque 1.077. Monocyte-derived macrophages (M) were isolated by 1-hour adherence to plastic,^6,7 while nonadhering cells were applied to a T cell enrichment column (R&D Systems, Inc.). The purity of M and the purity of T cells were greater than 85% to 95% as estimated by flow cytometry with anti-CD14 IgG and anti-CD3 IgG, respectively. The T cell fraction . . . [Full Text of this Article]

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