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(Arteriosclerosis, Thrombosis, and Vascular Biology. 2002;22:1427.)
© 2002 American Heart Association, Inc.

Vascular Biology

Connexin37 Is the Major Connexin Expressed in the Media of Caudal Artery

Nicole M. Rummery; Haruyo Hickey; Gordon McGurk; Caryl E. Hill

From the Division of Neuroscience, John Curtin School of Medical Research, Australian National University, Canberra, Australia.

Correspondence to N.M. Rummery, Division of Neuroscience, JCSMR, Australian National University, GPO Box 334, ACT 2601, Australia. E-mail nicole.rummery{at}anu.edu.au

Objective— To determine the connexins (Cxs) involved in intercellular coupling within vascular muscle, the present study has quantified mRNA and protein expression for Cx37, Cx40, Cx43, and Cx45 in the caudal artery (CA) and thoracic aorta (ThA) of the rat.

Methods and Results— Real-time polymerase chain reaction and immunohistochemistry identified Cx37 as the most abundantly expressed Cx in the CA, with fine punctate staining observed in the media. Conversely, mRNA for Cx43 was 40-fold greater in the ThA than in the CA, with punctate staining in the endothelium and media of the ThA but confined to the endothelium in the CA. Western blotting confirmed the differences in the relative amounts of Cx43 between the 2 vessels. For both arteries, Cx45 was expressed to a lesser degree in the media but not in the endothelium, whereas Cx40 was found only in the endothelium. Cx37, Cx40, and Cx43 were expressed in the endothelium of both vessels, although the density of Cx40 plaques was significantly greater in the CA.

Conclusions— The demonstration of Cx37 as the dominant Cx in the media of the CA highlights the potential heterogeneity in Cx involvement in vascular smooth muscle.

Key Words: connexin • endothelium • smooth muscle • thoracic aorta • caudal artery

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