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(Arteriosclerosis, Thrombosis, and Vascular Biology. 2002;22:710.)
© 2002 American Heart Association, Inc.

Letters to the Editor

Expression of the Novel Scavenger Receptor SR-PSOX in Cultured Aortic Smooth Muscle Cells and Umbilical Endothelial Cells

Oliver Hofnagel; Birgit Luechtenborg; Gabriele Plenz; Horst Robenek

Institute for Arteriosclerosis Research
University of Muenster
Muenster, Germany

To the Editor:

In the November issue of Arteriosclerosis, Thrombosis, and Vascular Biology, Minami et al¹ demonstrated expression of the novel scavenger receptor for phosphatidylserine and oxidized lipoprotein (SR-PSOX) in lipid-laden macrophages accumulated in the intima of human atherosclerotic lesions. Because SR-PSOX seems to be identical to the membrane-anchored chemokine CXCL16,^2,3 which may play a dual role in inflammation and homeostasis, Minami et al¹ discussed the potential regulation of SR-PSOX by pro-inflammatory cytokines. Although the authors did not detect SR-PSOX in smooth muscle cells (SMCs) and endothelial cells (ECs), they did discuss the possible expression of SR-PSOX in these cell types. Until now, only the expression of the scavenger receptors SR-AI/II,⁴ CD36,⁵ and LOX-1⁶ in SMCs has been described.

In our studies on the formation of SMC-derived foam cells during atherogenesis, we have focused on the expression of scavenger receptors,⁷ including SR-PSOX, in SMCs and ECs. We have also investigated the influence of cytokines on the expression of SR-PSOX in SMCs. Reverse transcriptase–polymerase chain reaction (PCR; primers for human SR-PSOX: 5'-TACACGAGGTTCCAGCTCCT-3' and 5'-GGGGGCTGGT- AGGAAGTAAA-3', porcine SR-PSOX: 5'-TATGTGGAGGCAGCAG- TGAC-3' and 5'-CTGCAGGGTAGATGGCAGAT-3') was performed on total RNA from cultured human and porcine aortic SMCs and human umbilical vein endothelial cells (HUVECs). PCR was performed at 94°C (45 seconds), 58°C (60 seconds), and 72°C (60 seconds) for 20 to 40 cycles in the linear area of amplification. The sequences of SR-PSOX products were confirmed by sequence analysis. ß-Actin served as the internal standard.

Thus, reverse transcriptase–PCR demonstrated the expression of SR-PSOX mRNA in porcine and . . . [Full Text of this Article]

Noriaki Kume

Department of Geriatric Medicine
Graduate School of Medicine
Kyoto University
Kyoto, Japan

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