This Article Full Text Full Text (PDF) Data Supplement All Versions of this Article: 22/4/638    most recent 01.ATV.0000013023.11297.B2v1 Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fournier, N. Articles by Kalopissis, A.-D. Search for Related Content PubMed PubMed Citation Articles by Fournier, N. Articles by Kalopissis, A.-D. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB CHOLESTEROL Medline Plus Health Information Triglycerides Related Collections Genetically altered mice Lipid and lipoprotein metabolism

(Arteriosclerosis, Thrombosis, and Vascular Biology. 2002;22:638.)
© 2002 American Heart Association, Inc.

Atherosclerosis and Lipoproteins

Opposite Effects of Plasma From Human Apolipoprotein A-II Transgenic Mice on Cholesterol Efflux From J774 Macrophages and Fu5AH Hepatoma Cells

Natalie Fournier; Anne Cogny; Véronique Atger; Danièle Pastier; Dominique Goudouneche; Antonino Nicoletti; Nicole Moatti; Jean Chambaz; Jean-Louis Paul; Athina-Despina Kalopissis

From the Laboratoire de Biochimie (N.F., D.G., N.M., J.-L.P.), Faculté des Sciences Pharmaceutiques, Châtenay-Malabry; Laboratoire de Biochimie (N.F., A.C., V.A., N.M., J.-L.P.), Hôpital Européen Georges Pompidou, AP-HP, Paris; U505 INSERM (D.P., J.C., A.-D.K.), Université Pierre et Marie Curie, Centre de Recherche des Cordeliers, Paris; and U430 INSERM (A.N.), Hôpital Broussais, AP-HP, Paris, France.

Correspondence to Dr N. Fournier, Laboratoire de Biochimie, Faculté des Sciences Pharmaceutiques, 5 rue JB Clément, 92296 Châtenay-Malabry, France. E-mail natalie_fournier{at}yahoo.fr

Overexpression of human apolipoprotein A-II (hapo A-II) in transgenic mice (hAIItg mice) induced marked hypertriglyceridemia and low levels of plasma high density lipoprotein (HDL) with a high hapo A-II content. We sought to determine whether cholesterol efflux to plasma and HDL from these mice would be affected. In the Fu5AH cell system, plasma from hAIItg mice induced a markedly lower cholesterol efflux than did control plasma, in accordance with the dependence of efflux on HDL concentration. Moreover, HDLs from hAIItg mice were less effective acceptors than were control HDLs. In the J774 macrophage cell system, pretreatment with cAMP, which upregulates ATP binding cassette transporter 1, induced a marked increase in the efflux to hAIItg plasma as well as to purified hapo A-I and hapo A-II, whereas it had no effect on cholesterol efflux to control plasma. A strong positive correlation was established between percent cAMP stimulation of efflux and plasma hapo A-II concentration. The cAMP stimulation of efflux to hAIItg mouse plasma may be linked to the presence of pre-ß migrating HDL containing hapo A-II. Thus, despite lower HDL and apolipoprotein A-I contents, the increased ability of plasma from hAIItg mice to extract cholesterol from macrophage-like cells may have an antiatherogenic influence.

Key Words: human apolipoprotein A-II transgenic mice • Fu5AH rat hepatoma cells • J774 mouse macrophages • cholesterol efflux • ATP binding cassette transporter 1

This article has been cited by other articles:

				M. Wroblewska, B. Kortas-Stempak, A. Szutowicz, and T. Badzio Phospholipids mediated conversion of HDLs generates specific apoA-II pre-{beta} mobility particles J. Lipid Res., April 1, 2009; 50(4): 667 - 675. [Abstract] [Full Text] [PDF]

				G. D. Wool, C. A. Reardon, and G. S. Getz Apolipoprotein A-I mimetic peptide helix number and helix linker influence potentially anti-atherogenic properties J. Lipid Res., June 1, 2008; 49(6): 1268 - 1283. [Abstract] [Full Text] [PDF]

				M. P. Adorni, F. Zimetti, J. T. Billheimer, N. Wang, D. J. Rader, M. C. Phillips, and G. H. Rothblat The roles of different pathways in the release of cholesterol from macrophages J. Lipid Res., November 1, 2007; 48(11): 2453 - 2462. [Abstract] [Full Text] [PDF]

				F. Zimetti, G. K. Weibel, M. Duong, and G. H. Rothblat Measurement of cholesterol bidirectional flux between cells and lipoproteins J. Lipid Res., March 1, 2006; 47(3): 605 - 613. [Abstract] [Full Text] [PDF]

				N. Rotllan, V. Ribas, L. Calpe-Berdiel, J. M. Martin-Campos, F. Blanco-Vaca, and J. C. Escola-Gil Overexpression of Human Apolipoprotein A-II in Transgenic Mice Does Not Impair Macrophage-Specific Reverse Cholesterol Transport In Vivo Arterioscler Thromb Vasc Biol, September 1, 2005; 25(9): e128 - e132. [Abstract] [Full Text] [PDF]

				J. W. J. Beulens, A. Sierksma, A. van Tol, N. Fournier, T. van Gent, J-L. Paul, and H. F. J. Hendriks Moderate alcohol consumption increases cholesterol efflux mediated by ABCA1 J. Lipid Res., September 1, 2004; 45(9): 1716 - 1723. [Abstract] [Full Text] [PDF]

				D. Sauvaget, V. Chauffeton, S. Dugue-Pujol, A.-D. Kalopissis, I. Guillet-Deniau, F. Foufelle, J. Chambaz, A. Leturque, P. Cardot, and A. Ribeiro In Vitro Transcriptional Induction of the Human Apolipoprotein A-II Gene by Glucose Diabetes, March 1, 2004; 53(3): 672 - 678. [Abstract] [Full Text] [PDF]